Abcam, ab277481, Anti-Hepatitis B Surface Antigen antibody [5C3]

Size: 100µL
Mouse Monoclonal Hepatitis B Surface Antigen (Adw) antibody. Suitable for IHC-P and reacts with Hepatitis B virus samples.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:5C3,
Isotype:IgG2a,
Light chain type:kappa,
Carrier free:No,
Reacts with:Hepatitis B virus,
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:This antibody stains the cytoplasm of antigen-positive liver cells in patients with Type B viral hepatitis. This antibody reacts with the 'a' determinant present on the HBsAg subtypes ayw1, ayw2, ayw3, ayw4, ayr, adw2, adw4 and adr. It does not react with normal tissues.

Properties and Storage Information:
Form-Liquid, Purity-Tissue culture supernatant, Storage buffer-pH: 7.4 - 7.6Preservative: 0.1% Sodium azideConstituents: 98.9% Tris buffer, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Hepatitis B Surface Antigen also known as HBsAg is a protein associated with the hepatitis B virus (HBV). It has a molecular weight of approximately 24 kDa. HBsAg expresses itself on the surface of HBV particles and infected liver cells. The protein exists in various forms including spherical and filamentous particles. Expression of HBsAg serves as a marker of active HBV infection and is a target for immune response.
Biological function summary
The Hepatitis B Surface Antigen facilitates the virus's entry into hepatocytes by attaching to the host cell receptors. The antigen is known to associate with a protein complex on the viral envelope that plays a role in the virus's infectivity and replication efficiency. The presence of HBsAg in the bloodstream signifies an ongoing infection and can stimulate the production of antibodies hepatitis B which aim to neutralize the virus and clear infection.
Pathways
Hepatitis B surface antigen plays a critical role in the immune evasion strategies of hepatitis B virus. It modulates key pathways such as the host's innate immune response affecting interferon signaling. HBsAg interacts with the hepatocyte receptor NTCP during the viral lifecycle which allows for the successful entry and subsequent infection of liver cells. NTCP’s involvement highlights the interconnectedness of how HBV exploits host cellular pathways to propagate.
The hepatitis B surface antigen is primarily associated with hepatitis B infection and its related complications such as chronic hepatitis B and hepatocellular carcinoma. Chronic presence of HBsAg in the bloodstream indicates active infection increasing the risk of liver damage over time. This condition often involves liver proteins such as alpha-fetoprotein which is frequently elevated in hepatocellular carcinoma illustrating the progression from hepatitis B infection to serious liver disorders.