Abcam, ab2785, Anti-Galectin 3 antibody [A3A12]

Size: 100µg
Anti-Galectin 3 antibody [A3A12] (ab2785) is a mouse monoclonal antibody detecting Galectin 3 in Western Blot, IHC-P, ICC/IF . Suitable for Human, Mouse . - Over 70 publications - Trusted since 2003
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:A3A12,
Isotype:IgG1,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WB, IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human LGALS3.P17931,
Epitope:The epitope for this antibody has been mapped to the first 58 amino acids of Galectin 3.,
Specificity:By Western blot, this antibody detects an ~30 kDa protein representing Galectin 3 from Jurkat cells transfected with human Galectin 3. Immunohistochemical staining of Galectin 3 in rat olfactory bulb yields a pattern consistent with nuclear and plasma membrane staining.

Product details:
What is this antibody validated in?
Anti-Galectin 3 antibody [A3A12] (ab2785) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse samples.
What is the molecular weight of Galectin 3?
Anti-Galectin 3 [A3A12] (ab2785) specifically detects a band for Galectin 3 (UniProt: P17931) at a molecular weight of 26kDa.
Trusted by the scientific community
Anti-Galectin 3 [A3A12] (ab2785) was first used in a scientific publication in 2003 and has been cited over 70 times in peer-reviewed journals.
Reviewed by scientists
Anti-Galectin 3 [A3A12] (ab2785) has over 15 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-Preservative: 0.05% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Galectin-3 also known as LGALS3 is a protein belonging to the galectin family which are beta-galactoside-binding lectins. This protein has a molecular weight of about 30 kDa. It shows expression in various tissues including the heart liver and gastrointestinal tract. Galectin-3 plays a mechanical role in cell-cell adhesion cell-matrix interactions and modulating immune responses. Researchers often use galectin-3 ELISA kits to quantify its presence in different biological samples.
Biological function summary
Galectin-3 participates in cell growth regulation apoptosis and immune system modulation. Galectin-3 does not typically integrate into larger molecular complexes but it interacts with a variety of cell surface and extracellular matrix molecules. Its function influences processes like cell differentiation and angiogenesis where it can act as a regulator leveraging its ability to bind glycans on cell surfaces and matrix components.
Pathways
Galectin-3 contributes to the regulation of pathways such as the MAPK signaling pathway and the Wnt signaling pathway. In the MAPK pathway it influences cell proliferation and survival. Galectin-3 interacts with proteins like Β-catenin in the Wnt pathway assisting in transcriptional regulation and cell fate determination. These pathways are vital for maintaining cellular homeostasis and responding to extracellular signals.
Galectin-3 has a strong connection to cancer and fibrosis. Its role in cancer involves promoting tumor cell survival metastasis and angiogenesis where it interacts with proteins like integrins to facilitate tumor progression. In fibrosis galectin-3 contributes to fibrotic tissue formation especially in conditions like idiopathic pulmonary fibrosis and cardiac fibrosis often associated with proteins such as TGF-Β. Understanding these interactions helps in developing therapeutic strategies targeting galectin-3 in these diseases.