Abcam, ab2792, Anti-P4HB antibody [RL90]

Size: 100µL
Anti-P4HB antibody [RL90] (ab2792) is a mouse monoclonal antibody detecting P4HB in Western Blot, Flow Cytometry, IP, IHC-P, IHC-Fr, ICC/IF, ELISA, EM . Suitable for African green monkey, Dog, Hamster, Human, Monkey, Mouse, Pig, Rat . - Over 220 publications - Trusted since 2003
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:RL90,
Isotype:IgG2a,
Carrier free:No,
Reacts with:Mouse, Rat, Dog, Human, Monkey, Hamster, African green monkey, Pig,
Applications:Flow Cyt, ELISA, EM, FuncS (Neut/Block), IHC-Fr, ICC/IF, IHC-P, WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Native Full Length Protein corresponding to Rat P4hb.P04785

Product details:
What is this antibody validated in?
Anti-P4HB antibody [RL90] (ab2792) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA, Electron microscopy (EM) in African green monkey, Dog, Hamster, Human, Monkey, Mouse, Pig, Rat samples.
What is the molecular weight of P4HB?
Anti-P4HB [RL90] (ab2792) specifically detects a band for P4HB (UniProt: P04785) at a molecular weight of 58kDa.
Trusted by the scientific community
Anti-P4HB [RL90] (ab2792) was first used in a scientific publication in 2003 and has been cited over 220 times in peer-reviewed journals.
Reviewed by scientists
Anti-P4HB [RL90] (ab2792) has over 45 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.05% Sodium azideConstituents: PBS, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
P4HB is a protein known as Protein Disulfide Isomerase (PDI). It plays a significant role in catalyzing the formation of disulfide bonds. This protein helps to fold newly synthesized proteins by shuffling disulfide bonds. It has a molecular mass of around 57 kDa. P4HB is mainly expressed in the endoplasmic reticulum of cells. While its alternative name is ERp59 P4HB is integral to the protein folding machinery inside cells.
Biological function summary
P4HB functions in protein folding and assembly. By acting as a chaperone it protects proteins from misfolding and aggregation. P4HB operates as part of a larger multi-protein complex that assists in maintaining protein structure under stress conditions in the cell. Its activity ensures protein stability and proper cellular function important for cell viability and health.
Pathways
P4HB plays a central role in the unfolded protein response (UPR) and oxidative protein folding pathway. It interacts with proteins such as calnexin and calreticulin through its involvement in these pathways. During oxidative protein folding P4HB introduces disulfide bonds into nascent proteins while removing incorrect ones ensuring efficient protein quality control.
P4HB alteration has connections to diseases like cancer and neurodegenerative disorders. Its overexpression and activity can promote tumor growth by aiding cancerous cell survival through protein homeostasis. Moreover P4HB's association with amyloid precursor protein impacts the progression of Alzheimer's disease. These connections make P4HB a relevant target for research into therapeutic interventions.