Abcam, ab279410, Human IL-17C ELISA Kit

Size: 1 x 96Tests
Human IL-17C ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-17C with a sensitivity of 1.2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 1.2 pg/mL,
Range:9.38 - 600 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human IL-17C ELISA kit (ab279410) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human IL-17C protein in human serum, plasma and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-17C with 1.2 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
IL-17C, also known as CX2, is a secreted cytokine of IL-17 family. IL-17C binds to a heterodimer formed by IL-17RA and IL-17RE. IL-17C plays a crucial role in innate immunity of the epithelium, including to intestinal bacterial pathogens, in an autocrine manner. It stimulates the production of antibacterial peptides and proinflammatory molecules for host defense by signaling through the NF-kappa-B and MAPK pathways. IL-17C is up-regulated by bacterial stimuli, including by lipopolysaccharides in gut epithelial cell lines, and by various proinflammatory cytokines, including TNF, IL-1B and IL-17F. IL-17C acts synergistically with IL-22 in inducing the expression of antibacterial peptides, and with TNF and IL-1B in inducing DEFB2 from keratinocytes. Depending on the type of insult, IL-17C may have both protective and pathogenic properties, either by maintaining epithelial homeostasis after an inflammatory challenge or by promoting inflammatory phenotype. It plays an important role in the pathogenesis of inflammatory arthritis.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C