Abcam, ab279653, Anti-Ki67 antibody [B56]

Size: 20µg / 100µg / 1mg
Anti-Ki67 antibody [B56] (ab279653) is a mouse monoclonal antibody detecting Ki67 in Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF . Suitable for Human, Mouse, Rat . - KO validated for confirmed specificity - Over 20 publications
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:B56,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, ICC/IF, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Product Specifications
Anti-Ki67 antibody [B56] (ab279653) is a mouse recombinant monoclonal antibody and is validated for use in in human, mouse, rat samples.
Anti-Ki67 antibody [B56] (ab279653) specifically detects Ki67 (UniProt ID: P46013; Molecular weight: 359kDa) and is sold in a convenient trial size to enable initial testing (20 µg) and larger sizes for subsequent scaling up experiments (100 µg and 1 mg).
Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-Ki67 antibody [B56] (ab279653) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
The specificity of Anti-Ki67 antibody [B56] (ab279653) has been confirmed by testing in knockout samples.
Anti-Ki67 antibody [B56] (ab279653) has been cited over 29 times in peer reviewed journals and is trusted by the scientific community.
Related Products
Conjugation-ready, carrier free format available for antibody clone B56 -
ab279657
Antibody clone B56 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488 (
ab283242
ab283699
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Ki67 is a nuclear protein often referred to as MKI67 with a molecular weight of approximately 345 kDa. This protein is strongly associated with cell proliferation. Scientists commonly use Ki67 staining techniques to identify and quantify proliferating cells in tissues. Ki67 is expressed in the nucleus during active cell cycle phases—G1 S G2 and M—but not in resting cells in G0 phase. It is detectable through methods such as Ki67 immunofluorescence and Ki67 flow cytometry which are important for analyzing cell division.
Biological function summary
The Ki67 protein plays an essential role in cellular proliferation processes. It does not form part of a stable complex but interacts transiently with other cell cycle-related proteins. Research indicates that Ki67 maintains the structure of the perichromosomal layer during mitosis impacting chromosome separation. It is particularly active in tissues with high cell turnover such as bone marrow and lymphoid organs.
Pathways
Ki67 influences several key cellular pathways that control cell proliferation and differentiation. The protein acts within the regulation of the cell cycle and the PI3K/AKT signaling pathway important for cell growth and survival. Within these pathways Ki67 interacts with proteins like cyclin-dependent kinases (CDKs) which regulate transitions between different phases of the cell cycle.
Ki67 has significant implications in oncology and can be used as a biomarker for cancer prognosis. Its expression levels help determine the aggressiveness of tumors especially in breast cancer and prostate cancer. High Ki67 levels correlate with poor prognosis as it indicates rapid cell division. In cancer Ki67 associates with p53 a protein that regulates the cell cycle and function as a tumor suppressor.