Abcam, ab279719, Anti-Apolipoprotein E4 antibody [EPR24181-64] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Apolipoprotein E antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-Fr, Flow Cyt (Intra), IHC-P and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR24181-64,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:IHC-P, ICC/IF, Flow Cyt (Intra), IP, IHC-Fr, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab279719 is the carrier-free version of
ab279714
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Apolipoprotein E4 often called ApoE4 is a member of the apolipoprotein family and plays an important role in lipid metabolism. As a 34-kDa protein it associates with lipoprotein particles and mediates receptor binding and clearance of these complexes. ApoE4 expresses mainly in the liver and the brain where it facilitates cholesterol and lipid transport and homeostasis. Other important alternative names for this protein include apo E4 and E4 peptide which identify specific functions related to its diverse roles in the body.
Biological function summary
ApoE4 is an important player in lipid transport and uptake facilitating the redistribution of lipids among cells. It forms part of a critical complex involved in binding to low-density lipoprotein receptors (LDLR) and the related protein family enabling efficient lipid delivery to peripheral tissues. The interaction of ApoE4 with these receptors directly influences cellular lipid balance impacting important cellular functions. Additionally its polymorphic nature allows the isoforms E2 E3 and E4 to differ in their impacts on lipid transport and disease risk.
Pathways
ApoE4 engages in the lipid and cholesterol transport pathways prominently impacting the reverse cholesterol transport process and the regulation of plasma lipoprotein metabolism. It interacts with proteins such as LDLR and LRP1 influencing the removal of triglyceride-rich lipoproteins and modulating lipid levels in the circulation. ApoE4's involvement in these pathways highlights its role as a central regulator of lipid homeostasis.
ApoE4 is closely associated with Alzheimer’s disease and cardiovascular disease highlighting its significance beyond lipid transport. ApoE4's isoform uniquely elevates the risk for Alzheimer's disease by potentially altering amyloid-beta metabolism and interfering with tau-associated pathologies making it a target of interest in neurodegenerative research. Additionally individuals bearing the ApoE4 allele exhibit an increased susceptibility to cardiovascular disorders due to its impact on cholesterol transport and clearance often linking it with elevated blood cholesterol levels. Through these diseases ApoE4 connects with other proteins like amyloid precursor protein (APP) and tau in Alzheimer's disease illustrating the broader implications of its biological functions.