Abcam, ab279775, Phospho-EIF2S1 (S52) and Total EIF2S1 ELISA Kit

Size: 1 x 96Tests
Phospho-EIF2S1 (S52) and Total EIF2S1 ELISA Kit is a Semi-quantitative ELISA for the measurement of Phospho-EIF2S1 (S52) and Total EIF2S1 in Human in Cell/Tissue Extracts samples.
Key facts
Detection method:Colorimetric,
Sample types:Cell Lysate,
Reacts with:Human,
Assay type:Semi-quantitative,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Phospho-EIF2S1 (S52) and Total EIF2S1 ELISA Kit (ab279775) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated EIF2S1 (S52) protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an
in vitro
enzyme-linked immunosorbent assay for the measurement of human phospho-EIF2S1 and total EIF2S1. An anti-pan EIF2S1 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and EIF2S1 present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, biotinylated anti-phospho-eIF-2A (S52) antibody is added to detect phosphorylated EIF2S1. In the remaining wells, rabbit anti-pan-eIF-2A antibody is used to detect pan EIF2S1. After washing away unbound antibody, HRP-Streptavidin or HRP-conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of EIF2S1 (S52) or pan EIF2S1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
EIF2S1 also called eIF2α is a subunit of the eukaryotic translation initiation factor 2 (eIF2) complex. This protein plays an important role in the initiation of protein synthesis by regulating the binding of the initiator methionyl-tRNA to the 40S ribosomal subunit. Its molecular weight is approximately 36 kDa. EIF2S1 is widely expressed in various tissues providing a fundamental function in protein biosynthesis across different cell types.
Biological function summary
EIF2α acts as a regulatory component of the eIF2 complex which is central to initiating translation in eukaryotic cells. Phosphorylation of eIF2α leads to inhibition of the exchange of GDP to GTP on eIF2 effectively reducing global protein synthesis while allowing the translation of specific mRNAs involved in stress responses. The eIF2α forms part of the eIF2 complex working closely with other subunits to exert its regulatory functions.
Pathways
EIF2α is involved in the integrated stress response (ISR) and other protein synthesis pathways. The phosphorylation state of eIF2α is important in ISR linking it to proteins such as PERK and GCN2 which respond to various stress signals. Phospho-eIF2α plays a significant role in modulating the translation response to cellular stress ensuring cells can adapt or initiate apoptosis depending on the conditions.
EIF2α connects to diseases like Alzheimer's and diabetes. Alterations in phosphorylation of eIF2α are linked to endoplasmic reticulum stress in Alzheimer's affecting protein homeostasis. Connecting to diabetes dysregulated eIF2α signaling interferes with insulin synthesis and secretion. Proteins like ATF4 also intersect with eIF2α in these diseases highlighting eIF2α's involvement in critical pathological processes.