Abcam, ab279792, Phosphotyrosine ErbB4 / HER4 ELISA Kit

Size: 1 x 96Tests
Phosphotyrosine ErbB4 / HER4 ELISA Kit is a Semi-quantitative ELISA for the measurement of Phosphotyrosine ErbB4 / HER4 in Human in Cell/Tissue Extracts samples.
Key facts
Detection method:Colorimetric,
Sample types:Cell Lysate,
Reacts with:Human,
Assay type:Semi-quantitative,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Phosphotyrosine ErbB4 / HER4 ELISA Kit (ab279792) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated ErbB4 / HER4 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an
in vitro
enzyme-linked immunosorbent assay for the measurement of human phospho-ErbB4 / HER4. An anti-ErbB4 / HER4 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and phosphorylated and unphosphorylated ErbB4 / HER4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-phosphotyrosine antibody is used to detect only tyrosine-phosphorylated protein. After washing away unbound antibody, Streptavidin-Conjugated HRP is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of phospho-ErbB4 / HER4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
For research use only - not intended for diagnostic use.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ErbB4 also known as HER4 is a member of the ErbB family of receptor tyrosine kinases. It functions mechanically as a transmembrane protein that activates downstream signaling pathways upon ligand binding. This receptor has an approximate mass of 180 kDa. You can find ErbB4 mainly expressed in the heart brain and various epithelial tissues. The ErbB4 protein often interacts with ligands such as Neuregulins heregulin and others.
Biological function summary
ErbB4 has several roles including the regulation of cell proliferation differentiation migration and development. It functions as part of a receptor complex initiating a cascade of intracellular signaling that impacts various cellular functions. ErbB4 ELISA assays often help quantify interactions and activities of the receptor providing insights into its broader functionalities. The ErbB4 protein also contributes to neuronal development and the maintenance of cardiac tissue integrity.
Pathways
ErbB4 participates in the PI3K/AKT pathway and the MAPK/ERK pathway essential for cell survival and proliferation. These pathways involve a network of proteins including other ErbB family members such as EGFR ErbB2 (HER2) and ErbB3 (HER3) forming heterodimers that enhance and diversify the signaling outcomes. E200 and HF R are often referred to in literature related to pathway analysis of these complex interactions.
ErbB4 has links to several conditions notably certain cancers and schizophrenia. The connection to cancer arises due to its role in oncogenic signaling where it can form aberrant partnerships with proteins like ErbB2 which stimulates cell growth. In schizophrenia alterations in ErbB4 expression or function may affect neural signaling pathways leading to cognitive deficits. Understanding these relationships helps to target therapies and develop treatments involving anti HER HF R 500 and similar inhibitors.