Abcam, ab279832, Phosphotyrosine Insulin Receptor ELISA Kit

Size: 1 x 96Tests
Phosphotyrosine Insulin Receptor ELISA Kit is a Semi-quantitative ELISA for the measurement of Phosphotyrosine Insulin Receptor in Human in Cell/Tissue Extracts samples.
Key facts
Detection method:Colorimetric,
Sample types:Cell Lysate,
Reacts with:Human,
Assay type:Semi-quantitative,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Phosphotyrosine Insulin Receptor ELISA Kit (ab279832) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated Insulin Receptor protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an
in vitro
enzyme-linked immunosorbent assay for the measurement of human phospho-Insulin Receptor. An anti-pan Insulin Receptor antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and phosphorylated and unphosphorylated Insulin Receptor present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-phosphotyrosine antibody is used to detect only tyrosine-phosphorylated protein. After washing away unbound antibody, HRP-conjugated Streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of phospho-Insulin Receptor bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The insulin receptor (INSR) is a transmembrane glycoprotein that functions as a receptor for insulin. Known also as CD220 the receptor has a molecular weight of about 320 kDa. It is widely expressed in insulin-sensitive tissues such as liver muscle and adipose tissues. The insulin receptor consists of two α subunits and two β subunits forming a heterotetramer. These subunits are located outside and inside the cell membrane respectively. The α subunits bind insulin while the β subunits possess tyrosine kinase activity important for downstream signaling.
Biological function summary
Insulin receptor plays a role in facilitating the effects of insulin on glucose and lipid metabolism. When insulin a protein binds to the insulin receptor it undergoes a conformational change that activates its intrinsic kinase activity. This activation further leads to tyrosine phosphorylation of intracellular targets resulting in modulation of cellular functions. Insulin receptor also participates in the formation of signaling complexes through interaction with substrates like insulin receptor substrate (IRS) proteins which are important for the transmission of the insulin signal inside cells.
Pathways
Insulin receptor is central to the insulin signaling pathway and the mitogen-activated protein kinase (MAPK) pathway. Activation of the insulin receptor triggers the insulin signaling cascade which involves various proteins like PI3 kinase and Akt that contribute to glucose uptake and metabolism. In the MAPK pathway the insulin receptor influences gene expression related to cell growth and differentiation. These pathways intertwine with other hormone signaling systems and affect numerous physiological processes critical for maintaining metabolic homeostasis.
Aberrations in insulin receptor function are linked to insulin resistance and type 2 diabetes. Defective signaling through this receptor impairs glucose transport into cells contributing to hyperglycemia. Disorders in insulin receptor activity also relate to metabolic syndrome a cluster of conditions increasing the risk of heart disease. Proteins connected to these conditions include IRS proteins and PI3 kinase highlighting the integrated roles these components play in metabolic regulation. Developing anti-insulin receptor antibodies offers potential therapeutic interventions to modulate receptor function and manage these disorders effectively.