Abcam, ab279846, Phospho-SLC7A5/LAT1 (Y161) and Total SLC7A5/LAT1 ELISA Kit

Size: 1 x 96Tests
Phospho-SLC7A5/LAT1 (Y161) and Total SLC7A5/LAT1 ELISA Kit is a Semi-quantitative ELISA for the measurement of Phospho-SLC7A5/LAT1 (Y161) and Total SLC7A5/LAT1 in Mouse in Cell/Tissue Extracts samples.
Key facts
Detection method:Colorimetric,
Sample types:Cell Lysate,
Reacts with:Mouse,
Assay type:Semi-quantitative,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Phospho-SLC7A5/LAT1 (Y161) and Total SLC7A5/LAT1 ELISA Kit (ab279846) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated SLC7A5/LAT1 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an
in vitro
enzyme-linked immunosorbent assay for the measurement of human phospho-SLC7A5/LAT1 and total SLC7A5/LAT1. An anti-pan SLC7A5/LAT1 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and SLC7A5/LAT1 present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, mouse anti-phospho SLC7A5/LAT1 (Y161) antibody is added to detect phosphorylated SLC7A5/LAT1. In the remaining wells, mouse anti-pan-SLC7A5/LAT1 antibody is used to detect pan SLC7A5/LAT1. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SLC7A5/LAT1 (Y161) or pan SLC7A5/LAT1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The SLC7A5/LAT1 or LAT1 transporter also known as the large neutral amino acid transporter small subunit 1 plays an important role in facilitating the transport of large neutral amino acids across cell membranes. LAT1 is a heterodimer with a heavy chain known usually as SLC3A2 forming a functional transporter which is around 40-50 kDa in mass. LAT1 is highly expressed in tissues with high metabolic rates like the brain placenta and some cancer cells making it important for nutrient transport in these areas.
Biological function summary
The LAT1 transporter mediates the uptake of essential amino acids which are critical for protein synthesis and cellular metabolism. It functions as part of the LAT1-SLC3A2 heterodimer complex ensuring effective transport across the blood-brain barrier and into other metabolically active tissues. This activity supports cellular growth and proliferation underlining its importance in rapidly dividing cells such as in cancer.
Pathways
LAT1 plays a critical role in the mTOR signaling pathway which is central to regulating cell growth and metabolism in response to nutrient availability. LAT1's transport function influences the activation of mTORC1 by ensuring a steady supply of leucine an amino acid that activates this complex. LAT1 is also integral to the cross-pathway interaction with the insulin signaling pathway enhancing nutrient-dependent growth signals in conjunction with other transporters.
Abnormal LAT1 function links closely with cancer and neurological disorders. Its overexpression often marks various tumors where it supports cancer cell survival by sustaining amino acid supply often in conjunction with increased SLC3A2 expression. In neurological contexts LAT1 impairments can contribute to disorders involving neurotransmitter imbalances as it participates in amino acid transport critical for neurotransmitter synthesis.