Abcam, ab279917, Phospho-RSK2 (S386) and Total RSK2 ELISA Kit

Size: 1 x 96Tests
Phospho-RSK2 (S386) and Total RSK2 ELISA Kit is a Semi-quantitative ELISA for the measurement of Phospho-RSK2 (S386) and Total RSK2 in Human in Cell/Tissue Extracts samples.
Key facts
Detection method:Colorimetric,
Sample types:Cell Lysate,
Reacts with:Human,
Assay type:Semi-quantitative,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Phospho-RSK2 (S386) and Total RSK2 ELISA Kit (ab279917) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated RSK2 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an
in vitro
enzyme-linked immunosorbent assay for the measurement of human phospho-RSK2 (S386) and total RSK2. An anti-pan RSK2 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and RSK2 present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho RSK2 (S386) antibody is added to detect phosphorylated eIF4EBP1. In the remaining wells, rabbit anti-pan-RSK2 antibody is used to detect pan RSK2. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of RSK2 (S386) or pan RSK2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RSK2 also known as ribosomal S6 kinase 2 is a serine/threonine-protein kinase with a molecular mass of approximately 85 kDa. This protein is expressed in various tissues with significant levels in the brain lungs and muscles. Mechanically RSK2 phosphorylates a variety of substrates involved in cell cycle regulation transcription and growth linking extracellular signals to intracellular responses. It contains two non-identical kinase domains which interact and modulate its activity leading to changes in cellular dynamics.
Biological function summary
RSK2 influences cell proliferation growth and survival by acting as a bridge in signaling cascades downstream of the extracellular signal-regulated kinase (ERK) pathway. This protein is not part of a larger complex but it interacts with other proteins such as ERK1/2 and substrates like ribosomal protein S6. By modulating these interactions RSK2 helps control gene expression related to cellular growth and differentiation functions.
Pathways
Many cellular processes involve RSK2 particularly through the MAPK/ERK signaling and the PI3K/AKT pathways. It plays a significant role in transmitting signals that regulate cellular and metabolic responses linking external growth factors and hormones to appropriate cellular actions. RSK2 interacts closely with proteins like ERK1/2 within these pathways to facilitate its role in linking upstream signals to downstream effects impacting cellular growth and metabolic activity.
Disruptions in RSK2 function connect the protein to Coffin-Lowry syndrome a genetic disorder characterized by cognitive disability and skeletal abnormalities. Additionally altered RSK2 activity has implications in certain cancers as it affects cell proliferation and survival pathways. Proteins like ERK and mTOR which are key players in these pathways often show interaction with RSK2 in the context of these disorders highlighting its significant role in disease pathogenesis.