Abcam, ab280330, Recombinant human Apolipoprotein E3

Size: 10µg / 50µg / 100µg / 250µg
Recombinant human Apolipoprotein E3 (ApoE3) is a Human Full Length ApoE protein in the 19 to 317 aa range with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, mass spectrometry and HPLC. The predicted molecular weight of ab280330 recombinant protein is 34 kDa. - Save time and ensure accurate results - use our recombinant Apolipoprotein E protein (ApoE) as a control - Available in different sizes to fit your experimental needs
Key facts
Purity:>95% SDS-PAGE,
Endotoxin level:<0.005 EU/µg,
Expression system:HEK 293 cells,
Tags:Tag free,
Applications:SDS-PAGE, Mass Spec, HPLCSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P02649,
Animal free:Yes,
Carrier free:No,
Species:Human,
Reconstitution:Reconstitute in PBS,
Storage buffer:pH: 7.4Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

Product details:
Ensure the validity of your result using our recombinant human Apolipoprotein E3 (ApoE3) ab280330 as a control.
The ab280330 ApoE3 protein is sourced from HEK293 cells and can be used as a positive control in SDS-PAGE, mass spectrometry and HPLC.
Check out our protein gel staining guide for SDS-PAGE

Properties and Storage Information:
Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions-Ambient, Appropriate long-term storage conditions-Ambient

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Apolipoprotein E (ApoE) also known as apolipoprotein e or apoE is a major protein involved in lipid metabolism. It has an approximate molecular weight of 34 kDa. This protein is mainly produced in the liver and brain where it plays a critical role in transporting lipoproteins fat-soluble vitamins and cholesterol. ApoE exists in three common isoforms: ApoE2 ApoE3 and ApoE4 each having different impacts on lipid binding and metabolic processes. Scientists often use an ApoE ELISA kit to quantify this protein in various samples providing insights into its expression in health and disease.
Biological function summary
ApoE's role as a constituent of chylomicrons VLDL and HDL particles. ApoE mediates the binding internalization and catabolism of these lipoprotein particles facilitating their interaction with specific cell-surface receptors such as the LDL receptor. This protein operates as part of a complex that includes various other apolipoproteins and lipid molecules. The study of mouse apoe using tools like a mouse apoe ELISA provides valuable data due to its similar physiological functions in lipid transport and metabolism.
Pathways
In the lipid metabolism pathway ApoE interacts with proteins such as the LDL receptor influencing the clearance of chylomicron remnants and VLDL from the bloodstream. In the cardiovascular disease pathway this protein impacts cholesterol levels and promotes plaques stabilization. ApoE's role in these pathways offers insights into its interaction with related proteins like apolipoprotein B and LDL receptor which are critical for maintaining lipid equilibrium.
In Alzheimer's disease ApoE4 isoform has a higher risk factor compared to ApoE3 and ApoE2 contributing to amyloid plaque formation through interactions with amyloid precursor protein. In cardiovascular diseases ApoE abnormalities influence atherosclerosis development with ApoE-deficient models showing increased susceptibility. ApoE's links to these diseases also connect it to other key proteins such as presenilin-1 in Alzheimer's disease and apolipoprotein B in cardiovascular disorders highlighting its extensive biological impact.