Abcam, ab280363, Anti-LATS2 antibody [EPR23126-488] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal LATS2 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Synthetic peptide - Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR23126-488,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab280363 is the carrier-free version of
ab243657
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
LATS2 also known as Large Tumor Suppressor kinase 2 is a serine/threonine-protein kinase with a molecular mass of approximately 121 kDa. It functions as a component of the Hippo signaling pathway where it helps regulate cell proliferation apoptosis and organ size. LATS2 shows expression in various tissues with notable levels in the lung heart and liver. Its role in these tissues makes it an important target for research in cancer and developmental biology.
Biological function summary
LATS2 plays an important role in cell cycle regulation and tumor suppression. LATS2 forms part of the core kinase complex within the Hippo pathway working closely with other proteins like MST1/2 kinases and Scaffold proteins SAV1. Through phosphorylation LATS2 can inhibit the activity of YAP and TAZ transcription co-activators promoting cell contact inhibition and proper tissue architecture. Its activity is important for limiting excessive cell growth and ensuring accurate cell division.
Pathways
LATS2 integrates into the Hippo signaling pathway and influences the PI3K-AKT signaling pathway. Within the Hippo pathway LATS2 interacts with MST1/2 forming an upstream kinase complex activating through phosphorylation of downstream transcription co-activators YAP and TAZ. These interactions result in the transcriptional regulation of genes controlling cell growth and apoptosis. Overlap with the PI3K-AKT pathway links LATS2 to cellular processes related to growth migration and survival providing a critical nexus that communicates cell status and environmental cues.
LATS2 has associations with both cancer and cardiac hypertrophy. In cancer dysregulation of LATS2 activity can result in aberrant cell growth and tumor development often noted in different types of carcinomas where it can act as a tumor suppressor. Mutations or reduced expression of LATS2 can correlate with increased cancer progression implying a need for comprehensive understanding of its regulation and function. Additionally in cardiac hypertrophy LATS2 contributes to cardiac cell size regulation and its imbalance can connect to abnormal heart muscle thickening. In these contexts LATS2 interaction with proteins such as YAP and TAZ provides an important point of interest for therapeutic interventions.