Abcam, ab280938, Native human Neutrophil Elastase protein (Active)

Size: 100µg
Native human Neutrophil Elastase protein (Active) is a Human Full Length protein, in the 30 to 267 aa range, expressed in Native, with >95%, suitable for SDS-PAGE, FuncS, ELISA.
Key facts
Purity:>95% SDS-PAGE,
Expression system:Native,
Tags:Tag free,
Applications:SDS-PAGE, ELISA, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:1 unit hydrolyzes one μmole per minute of MeO-Suc-Ala-Ala-Pro-Val-pNA at 25°C, in 100mM Tris-Hcl, pH 7.5 with 500mM NaCl.Specific activity = ≥20 U/mg protein.,
Accession:P08246,
Animal free:No,
Carrier free:Yes,
Species:Human,
Storage buffer:pH: 5.5Constituents: 0.87% Sodium chloride, 0.41% Sodium acetate

Product details:
ab280938 was prepared from whole blood shown to be non-reactive for HbsAG, anti-HCV, anti HBc, and negative for HIV 1 & 2 by FDA approved tests.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Neutrophil Elastase also known as neutrophil proteinase 3 has a molecular mass of approximately 30 kDa. It is a serine protease expressed mainly in azurophilic granules of neutrophils. This enzyme breaks down various proteins including elastin at sites of inflammation. Neutrophil Elastase releases during degranulation or through neutrophil extracellular traps (NETs) to combat pathogens.
Biological function summary
The target plays a significant role in neutrophil-mediated tissue remodeling and host defense. It leads to the breaking down of extracellular matrix components contributing to tissue remodeling and repair. Neutrophil Elastase is part of a complex interplay with other proteases like matrix metalloproteinases (MMPs). Its activity is regulated by endogenous inhibitors such as alpha-1 antitrypsin to prevent excessive tissue damage.
Pathways
This protease is important in inflammation and immune response pathways. It plays a role in the complement system by activating C5 a component of the complement cascade. It also acts in conjunction with other proteases such as cathepsin G to modulate immune and inflammatory responses. Neutrophil Elastase's role within these pathways is pivotal for the regulation of inflammation and destruction of pathogens.
Individuals with conditions such as chronic obstructive pulmonary disease (COPD) and cystic fibrosis often exhibit dysregulation or excessive activity of Neutrophil Elastase. This excessive activity leads to tissue damage and exacerbates disease progression. The enzyme also interacts with proteins like elastin and collagen in these diseases further contributing to pathological tissue remodeling and damage.