Abcam, ab2811, Anti-KPNB1 antibody [3E9]

Size: 100µL
Anti-KPNB1 antibody [3E9] (ab2811) is a mouse monoclonal antibody detecting KPNB1 in Western Blot, Flow Cytometry, IP, ICC/IF . Suitable for Human, Mouse, Rat . - Over 60 publications - Trusted since 2003
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:3E9,
Isotype:IgG2a,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:Flow Cyt, ICC/IF, WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Product details:
What is this antibody validated in?
Anti-KPNB1 antibody [3E9] (ab2811) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of KPNB1?
Anti-KPNB1 [3E9] (ab2811) specifically detects a band for KPNB1 (UniProt: Q14974) at a molecular weight of 97kDa.
Trusted by the scientific community
Anti-KPNB1 [3E9] (ab2811) was first used in a scientific publication in 2003 and has been cited over 60 times in peer-reviewed journals.
Reviewed by scientists
Anti-KPNB1 [3E9] (ab2811) has over 15 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.05% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
KPNB1 also known as importin beta plays hard role in nucleocytoplasmic transport. This large protein with a mass approximately 97 kDa shuttles proteins and ribonucleoproteins into the nucleus. KPNB1 expression occurs widely in many tissues reflecting its essential function in cell operations. As a solenoid protein KPNB1 structure allows flexibility needed for diverse interactions with cargo proteins and nuclear pore complexes.
Biological function summary
Importin beta facilitates the import of nuclear localization signal (NLS)-bearing proteins into the nucleus. KPNB1 forms a complex with importin alpha to mediate import processes. Importin alpha binds to the NLS-containing cargo while KPNB1 interacts with the nuclear pore complex. This system oversees important regulation of several cellular processes including signal transduction and cell cycle progression. The protein's role helps to organize cellular response to various stimuli maintaining cellular homeostasis.
Pathways
KPNB1 contributes to regulation in the nuclear import and export pathways. It tightly associates with Ran-GTPase a protein involved in the Ran pathway. This interaction supports directionality and specificity of energy-dependent nuclear transport. Additionally KPNB1 associates with proteins like NUP50 and NUP98 functioning as a gatekeeper at the nuclear pore complex by controlling cargo passage. Through these pathways KPNB1 balances cellular imports and exports critical for cell health and functionality.
KPNB1 has links to cancer notably in tumorigenesis and cancer progression. Studies show aberrant KPNB1 expression affects the nuclear import machinery supporting cancer cell proliferation and survival. KPNB1 interacts with oncogenic proteins such as c-Myc influencing transcription regulation in tumor cells. Furthermore KPNB1 holds relevance in neurodegenerative diseases like Alzheimer's where impaired transport function contributes to pathological accumulation of proteins in neuronal cells.