Abcam, ab28141, Anti-Rex1 antibody

Size: 100µg
Rabbit Polyclonal Rex1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 15 publications.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Rex1 also known as Zfp42 is a transcription factor and zinc finger protein with a mass of approximately 42 kDa. This protein is expressed mostly in embryonic stem cells and some adult stem cells. Rex1 functions by binding to DNA sequences influencing gene expression that is essential for maintaining stem cell self-renewal and pluripotency. As a result Rex1 plays an important role in regulating cellular processes related to developmental potential.
Biological function summary
Rex1 influences stem cell pluripotency and differentiation. It is part of the network of pluripotency factors along with Oct4 Sox2 and Nanog. These proteins form a regulatory circuit that maintains embryonic stem cell identity. Rex1 directly interacts with chromatin structure facilitating the accessibility of specific genes related to stem cell maintenance and identity. The expression levels of Rex1 have a significant impact on the transition between pluripotency and differentiated states within cells.
Pathways
Rex1 is involved in key signaling pathways regulating pluripotency like the LIF/STAT3 and Wnt signaling pathways. Within these pathways Rex1 collaborates with other transcription factors to promote the expression of genes required for stem cell growth and maintenance. Rex1 and Nanog work closely in these pathways to sustain the pluripotent status of stem cells by activating necessary genetic programs and preventing differentiation signals.
Rex1 has connections to certain cancers such as teratocarcinomas due to its role in maintaining pluripotency. Another protein Oct4 often has elevated levels alongside Rex1 in these cancerous cells which drives the tumor's stem-like characteristics. Rex1 levels have been associated with tumor formation and progression in some cancer types suggesting that it could be a biomarker for cancer stem cells and a potential target for therapies.