Abcam, ab282117, Anti-Synaptopodin antibody [EPR23922-79] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Synaptopodin antibody. Carrier free. Suitable for IP, WB, IHC-Fr, IHC-P and reacts with Mouse, Rat, Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR23922-79,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:IP, WB, IHC-Fr, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab282117 is the carrier-free version of
ab259976
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Synaptopodin also known as SYNPO is an actin-associated protein with a molecular mass of approximately 100 kDa. It plays a mechanical role by associating and bundling actin filaments important for cytoskeletal dynamics. Synaptopodin gets expressed mainly in podocytes of the kidney as well as in certain neurons particularly found in dendritic spines and telencephalic dendrites. This protein is sensitive to calcium signaling which influences its interaction with the actin cytoskeleton.
Biological function summary
The actin-modulating effects of Synaptopodin contribute to the regulation of cell shape and motility. Synaptopodin is essential for the formation of the dendritic spine apparatus working as a part of a protein complex that stabilizes dendritic spines which are key to synaptic plasticity and signal transduction. The protein can also influence the podocyte function in the kidney maintaining the structure of foot processes that are fundamental for the kidney filtration barrier.
Pathways
Synaptopodin plays an important role in the maintenance of synaptic plasticity and the actin cytoskeleton pathway which affects both neuronal plasticity and kidney function. Within these pathways Synaptopodin interacts with proteins like alpha-actinin and myosin-Va forming linkages that stabilize actin filaments and regulate cytoskeletal organization. The protein contributes to the protection of neurons and kidney glomeruli through signaling pathways that affect actin dynamic balance.
Synaptopodin has significant implications in nephrotic syndrome and Alzheimer's disease. In nephrotic syndrome alterations in Synaptopodin expression or function can lead to podocyte injury contributing to kidney dysfunction. In Alzheimer's disease Synaptopodin modulation of spine morphology may impact synaptic connectivity and cognitive function. The protein interacts with nephrin in kidney pathology and potentially affects tau protein signaling in Alzheimer's illustrating its vital role in both neurological and renal health.