Abcam, ab282287, Anti-ProCathepsin B antibody [EPR24353-25] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Cathepsin B antibody. Carrier free. Suitable for Flow Cyt (Intra), WB, IHC-P and reacts with Mouse, Human, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR24353-25,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Rat, Human, Mouse,
Applications:Flow Cyt (Intra), WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab282287 is the carrier-free version of
ab270998
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ProCathepsin B also known as Cathepsin B precursor or CTSB is a cysteine protease with a mature form mass of around 37 kDa. This protein begins as an inactive zymogen requiring processing to become active. It expresses mainly in lysosomes but can also be found extracellularly particularly in disease states. Typically abundant in various tissues ProCathepsin B undergoes proteolytic activation by removal of its propeptide region to function effectively in proteolysis.
Biological function summary
ProCathepsin B facilitates protein catabolism by degrading extracellular matrix components. It does not commonly form part of a larger protein complex allowing it to perform its proteolytic activity independently. The protein's actions are important for cellular turnover and tissue remodeling. In healthy tissues ProCathepsin B supports normal cellular homeostasis but dysregulation can lead to pathological conditions.
Pathways
ProCathepsin B plays a role in the proteolytic pathways involving processes such as autophagy and apoptosis. It participates in the ubiquitin-proteasome pathway interrelated with proteins like Ubiquitin and Caspases. These pathways control protein degradation and programmed cell death highlighting ProCathepsin B's importance in maintaining cellular function and regulation.
ProCathepsin B is associated with conditions like cancer and arthritis. In cancer its overexpression facilitates tumor invasion and metastasis often linked with proteins such as Matrix Metalloproteinases (MMPs). In arthritis increased ProCathepsin B activity contributes to joint tissue breakdown connecting with Collagen too. These associations make ProCathepsin B a potential target for therapeutic intervention in such disorders.