Abcam, ab282605, Anti-Rac1 + Rac2 + Rac3 antibody [102/Rac1] - BSA and Azide free

Size: 100µg / 1mg
Mouse Recombinant Monoclonal Rac1 + Rac2 + Rac3 antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat, Human samples.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:102/Rac1,
Isotype:IgG2b,
Light chain type:kappa,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Product details:
ab282605 is the carrier-free version of
ab282581
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Rac1 Rac2 and Rac3 are small GTPases belonging to the Rho family of proteins often referred to as Rac GTPases. These proteins are involved mechanically in the regulation of cell growth cytoskeletal reorganization and the generation of reactive oxygen species. Rac1 has a molecular mass of approximately 21 kDa and Rac proteins share a highly conserved amino acid sequence. Rac1 is ubiquitously expressed while Rac2 is mostly found in hematopoietic cells and Rac3 is expressed in the brain and other tissues.
Biological function summary
Rac proteins play key roles in cell movement adhesion and proliferation. Rac1 Rac2 and Rac3 participate in the formation of lamellipodia which are cellular protrusions important for migration. These proteins are part of complexes involved in actin cytoskeleton dynamics affecting how cells interact with their environment. Different Rac isoforms contribute to the precise control and timing of cellular responses to external stimuli influencing processes such as immune cell function and neuronal development.
Pathways
The Rac proteins integrate into the signaling processes that control actin organization and cell cycle progression. The Rac GTPases interact with pathways such as the PI3K/Akt and MAPK pathways. In these pathways they partner with proteins like PAK (p21-activated kinase) to facilitate changes in the cytoskeleton and promote survival signals. These interactions allow cells to modify their shape and migrate in response to different stimuli enabling a connection between cell signaling and movement.
Rac proteins link to several pathological conditions including cancer and immune-related disorders. Rac1 is often implicated in cancer where its activity affects tumor growth and metastasis through alterations in the cell cycle and motility. Rac2 mutations can lead to immune system dysfunctions impacting immune cell migration and response contributing to conditions like chronic granulomatous disease. The dysregulation of Rac activity often involves interactions with other protein partners like Nox enzymes which are responsible for reactive oxygen species production playing a part in disease progression.