Abcam, ab283654, Anti-CD68 antibody [EPR23917-164]

Size: 20µL / 100µL / 1mL
Anti-CD68 antibody [EPR23917-164] (ab283654) is a rabbit monoclonal antibody detecting CD68 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, IHC-Fr, ICC/IF, mIHC . Suitable for Mouse, Rat . - KO validated for confirmed specificity - Multiplex IHC validated on the Leica BOND® MAX using Opal reagents - Biophysical QC for unrivalled batch-batch consistency - Over 40 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR23917-164,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat,
Applications:ICC/IF, IHC-P, Flow Cyt (Intra), WB, mIHC, IHC-FrSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Mouse unsuitable for IHC-Fr application

Product details:
Anti-CD68 antibody [EPR23917-164] (ab283654) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-Fr, IHC-P and WB.
Abcam's high quality manufacturing and validation processes ensure Anti-CD68 antibody [EPR23917-164] (ab283654) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
The specificity of Anti-CD68 antibody [EPR23917-164] (ab283654) has been confirmed by Western Blot testing in CD68 knockout RAW 264.7 cells (cell lysate
ab280106
Anti-CD68 antibody [EPR23917-164] (ab283654) specifically detects CD68 (UniProt ID: P31996; Molecular weight: 33kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).
Conjugation-ready, carrier free format available for antibody clone EPR23917-164 -
ab283667
Antibody clone EPR23917-164 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor
647, Alexa Fluor
594, APC, Alexa Fluor
ab305214
ab312903
ab317758
ab319086
CD68 levels is implicated in autoimmune conditions like rheumatoid arthritis, where macrophages contribute to joint inflammation and damage.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Shipping conditions update: ambient shipping
This product will be delivered at ambient temperature instead of chilled – this is by design. Extensive stability testing confirmed that our products are suitable for shipment under ambient conditions and maintain expected quality.
Why the change?
It’s part of our commitment to more sustainable packaging solutions, with ambient deliveries using eco-friendly materials such as recyclable cardboard instead of polystyrene.
What you need to know
Ambient shipments come clearly marked on the delivery note.
No ice will be included in ambient shipments, but mixed orders (ambient and cold-chain items) will still arrive with ice packs to protect temperature-sensitive products.
Warranty coverage remains fully valid, aligned with our validated shipping method.
Please store the product as per the datasheet instructions upon receipt.
Find out more -
https://www.abcam.com/en-us/support/shipping-storage-support/ambient-shipping

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Conditional Ambient, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CD68 is a glycoprotein that functions as a scavenger receptor. It plays an important role in the regulation of cellular debris and apoptotic cell clearance. Researchers often refer to it as KP1 or macrosialin in the literature. CD68 with a molecular weight of approximately 110 kDa is highly expressed in macrophages and monocytes. The mucin-like structure of CD68 allows it to be heavily glycosylated which aids in its function. Researchers detect CD68 in tissues through methods like immunohistochemistry (IHC) immunofluorescence and CD68 staining and it is widely used as a macrophage marker in research.
Biological function summary
CD68 facilitates functions associated with the innate immune response. It serves as an important part of macrophages which contribute to immune surveillance and tissue homeostasis. Though it primarily operates on its own in some contexts CD68 might assist in forming complexes with other receptors to modulate phagocytic activity. This target is significant in atherosclerotic plaque stability as macrophages engulf lipids and cell debris through processes facilitated by CD68.
Pathways
CD68 is involved in pathways connected to inflammation and phagocytosis. The CD68 protein works closely with other scavenger receptors like CD36 to mediate uptake of oxidized low-density lipoproteins (oxLDL) in the lipid metabolism pathway. Additionally CD68 engagement can influence the toll-like receptor (TLR) signaling pathways thereby linking innate immunity and inflammatory responses critical for host defense and disease progression.
CD68 is associated with atherosclerosis and multiple sclerosis. In atherosclerosis its role becomes evident as it accumulates in macrophages within the plaques making it a marker of disease severity. In multiple sclerosis CD68 expression in macrophages and microglia indicates active demyelination and inflammation. It also relates to proteins such as CD36 in atherosclerosis where both mediate the uptake of modified LDL contributing to foam cell formation in plaques.