Abcam, ab283884, Mouse FGL2/Prothrombinase ELISA Kit

Size: 1 x 96Tests
Mouse FGL2/Prothrombinase ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse FGL2/Prothrombinase with a sensitivity of 0.03 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.03 ng/mL,
Range:0.188 - 12 ng/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
MouseFGL2/Prothrombinase ELISA Kit (ab283884) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FGL2/Prothrombinase protein in serum, plasma, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse FGL2/Prothrombinase with 0.03 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
FGL2 (Fibrinogen-Like Protein 2, also known as Fibroleukin and Prothrombinase exists as both membrane bound and secreted by cytotoxic and helper T-cells (CD8+ and CD4+). By converting prothrombin to thrombin, it contributes to physiologic lymphocyte functions at mucosal sites.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
FGL2 also known as Fibroleukin or Prothrombinase is a protein involved in various physiological and pathological processes. Its molecular mass is approximately 70 kDa. This protein is widely expressed in different tissues including the liver and the spleen as well as in immune cells such as T cells and macrophages. It serves as an important signaling molecule and mediator in various biological pathways due to its capacity to interact with other proteins and molecular complexes.
Biological function summary
FGL2 acts in immune response modulation and blood coagulation. It can exist as a homodimer or form part of a larger protein complex. One of its roles involves cleaving prothrombin to generate thrombin contributing to the coagulation cascade. Moreover FGL2 has immunosuppressive properties affecting T cell proliferation and cytokine release. Its dual function in coagulation and immune regulation points to its importance in maintaining homeostasis and orchestrating immune responses.
Pathways
FGL2 serves key roles in both the coagulation and immune pathways. In the coagulation pathway FGL2 aids in the conversion of prothrombin to thrombin bridging innate immunity with coagulation processes. This action places it in close functional relationship with proteins in the coagulation pathway such as thrombin and fibrin. Moreover its involvement in immune pathways aligns it with cytokines and interferons modulating the immune response and maintaining immune system balance.
FGL2 plays significant roles in conditions such as viral hepatitis and certain autoimmune disorders. In viral hepatitis FGL2's upregulation contributes to liver fibrosis and inflammation often in conjunction with another protein CD147. In autoimmune disorders the dysregulation of FGL2 can lead to excessive inflammatory responses impacting proteins like the interferon-gamma. Understanding FGL2's involvement in these diseases can inform therapeutic strategies targeting both coagulation and immune dysregulation.