Abcam, ab283914, Anti-SARS-CoV2 orf8 antibody [EPR24851-65]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal SARS-CoV2 orf8 antibody. Suitable for ELISA, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with SARS-CoV-2, Transfected cell line - SARS-CoV-2 samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR24851-65,
Isotype:IgG,
Carrier free:No,
Reacts with:SARS-CoV-2,
Applications:ELISA, WB, IHC-P, Flow Cyt (Intra), ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SARS-CoV-2 Orf8 also known as open reading frame 8 is a protein of interest in studying the SARS-CoV-2 virus. It is a viral accessory protein that weighs around 22 kDa and is expressed abundantly in infected host cells especially within the endoplasmic reticulum. Researchers identify this protein as having a unique location among Coronaviridae setting it apart in function when compared to other viral proteins.
Biological function summary
The Orf8 protein interacts with host cellular machinery to affect immune response. It is not part of any known stable complexes but it modulates the unfolded protein response (UPR) in cells influencing how proteins fold and maintaining cellular homeostasis under stress conditions. Orf8 can also impact the major histocompatibility complex class I (MHC-I) pathway which has implications on immune evasion by the virus.
Pathways
Studies indicate that SARS-CoV-2 Orf8 plays roles in immune modulation and protein processing pathways. It interacts with the UPR pathway notably affecting the IRE1 signaling. Comparisons to other viral accessory proteins such as SARS-CoV-2 Orf3b reveal distinct impacts on host immune responses illustrating the diverse strategies the virus employs to enhance survival and replication.
Orf8's expression links with COVID-19 severity contributing to immune dysregulation seen in patients. It has connections to inflammatory disorders due to its interference with the MHC-I pathway. Researchers study Orf8 alongside Nsp1 to understand the synergistic effects in manipulating host immune responses aiding in the pathogenesis of the disease. Understanding Orf8 can provide insights into targeted therapeutic interventions for COVID-19.