Abcam, ab284002, Human GRX2 ELISA Kit

Size: 1 x 96Tests
Human GRX2 ELISA Kit is a Sandwich (qualitative) ELISA for the measurement of Human GRX2 in Human in Cell/Tissue Extracts samples.
Key facts
Detection method:Colorimetric,
Sample types:Cell Lysate,
Reacts with:Human,
Assay type:Sandwich (qualitative),
Sensitivity:= 0.3 ng/mL,
Range:0.038 - 2.4 ng/mL,
Assay time:4h,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human GRX2 ELISA Kit (ab284002) is designed for detection of glutaredoxin-2 in human cell lysate samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human glutaredoxin-2 in approximately 4 hours.
A polyclonal antibody specific for human glutaredoxin-2 has been pre-coated onto a 96-well microplate with removable strips. Glutaredoxin-2 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human glutaredoxin-2, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The target GRX2 also commonly known as GLRX2 is a human protein encoded by the GLRX2 gene. GRX2 functions as a glutaredoxin a type of enzyme involved in the process of reducing disulfide bonds in proteins which is essential for cell redox regulation. The protein has a molecular mass of approximately 16 kDa and primarily resides in the mitochondria and cytoplasm of cells. Its expression occurs in various tissues with significant levels in the heart brain and skeletal muscle indicating its broad functional roles across different organ systems.
Biological function summary
The GRX2 protein plays a critical role in maintaining cellular redox balance by facilitating the reversible oxidation of protein cysteines. This is important for protecting cells against oxidative stress. GRX2 does not function as an isolated entity but instead forms part of larger multi-protein complexes interacting with other enzymes to regulate cellular homeostasis. These interactions help to protect cells from oxidative damage promote protein folding and regenerate other antioxidants.
Pathways
GRX2 holds a significant position in the redox regulation and antioxidant pathways. It partners with thioredoxin reductase and glutathione systems to mediate these pathways and manage cellular oxidative stress. The protein's activity is closely tied to glutathione with GRX2 often acting in synergy with glutathione to reduce complex oxidized protein thiols. This collaboration is vital in the oxidative stress response where the pathway elements must efficiently counterbalance oxidative challenges.
GRX2's function links it to several oxidative stress-related conditions including neurodegenerative diseases and cardiovascular disorders. In neurodegenerative diseases like Parkinson's disease GRX2's ability to combat oxidative stress is compromised leading to neuronal damage. Additionally the protein's interaction with other redox regulatory proteins such as thioredoxin suggests interconnected roles in the progression of these disorders. Cardiovascular diseases also involve GRX2 due to its essential function in maintaining cardiovascular integrity under environmental and metabolic oxidative stress.