Abcam, ab284615, Human Complement Factor I ELISA Kit

Size: 1 x 96Tests
Human Complement Factor I ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Complement Factor I with a sensitivity of 0.17 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Cell culture media, Heparin Plasma, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.17 ng/mL,
Range:0.313 - 20 ng/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Complement Factor I ELISA Kit ab284615 is a rapid single-wash 90-min Sandwich ELISA to measure Human Complement Factor I in cell culture media, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 0.17 ng/mL.
How the assay works
Human Complement Factor I SimpleStep ELISA
employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA
kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human Complement Factor I ELISA Kit ab284615 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Complement Factor I (CFI) also known as C3b/C4b inactivator Complement Factor I and Factor I Complement is a serine protease with a mass of approximately 88 kDa. It is expressed in the liver and secreted into the bloodstream. This protein cleaves complement components C3b and C4b preventing the assembly of C3/C5 convertase. As a regulator CFI ensures the complement system does not damage host tissues by maintaining it in check.
Biological function summary
Complement Factor I plays a significant role in controlling complement activation. It operates as part of a regulatory complex with cofactors that include Factor H C4-binding protein and membrane cofactor protein (MCP/CD46). By inactivating C3b and C4b fragments CFI helps prevent the formation of the terminal complement complex offering protection against excessive immune response and potential tissue damage. This regulation is essential for maintaining homeostasis within the immune system.
Pathways
Complement Factor I is involved in both the alternative and classical pathways of complement activation. It relates closely to proteins like Factor H and C3 convertase as its actions are vital in the degradation of C3b to iC3b and C4b to inactive forms. This regulatory mechanism ensures proper immune surveillance and prevention of autoimmune reactions by controlling the extent of complement activation on cell surfaces.
Deficiencies or mutations in Complement Factor I are connected to conditions such as atypical hemolytic uremic syndrome (aHUS) and age-related macular degeneration (AMD). These disorders arise due to dysregulation of the complement system where CFI links to proteins like Factor H and C3 impacting disease progression. Proper function of CFI in these diseases serves to protect tissues from the immune overactivation that leads to disease symptoms.