Abcam, ab284701, Anti-PPP1CB antibody [EP1804Y] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal PPP1CB antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP1804Y,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Human, Rat,
Applications:Flow Cyt (Intra), IP, ICC/IF, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab284701 is the carrier-free version of
ab53315
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PPP1CB also known as Protein Phosphatase 1 Catalytic Subunit Beta is a member of the catalytic subunit of Protein Phosphatase 1 (PP1). It has an approximate mass of 37 kDa. PPP1CB is expressed in various tissues throughout the human body including the heart brain and skeletal muscle. This phosphatase is primarily involved in the dephosphorylation of proteins a critical function to regulate cellular processes. As a catalytic subunit PPP1CB mediates various signaling cascades by removing phosphate groups from target proteins.
Biological function summary
PPP1CB plays key roles in cell division signal transduction and muscle contraction. It acts as part of a larger complex interacting with a variety of regulatory proteins and substrates to fine-tune cellular activities. Through dephosphorylation PPP1CB aids in the transition between different phases of cell cycles impacting cellular growth and proliferation. It is also involved in glycogen metabolism where it removes phosphate groups from glycogen phosphorylase ensuring proper energy balance in cells.
Pathways
PPP1CB participates significantly in the TGF-beta signaling and Wnt signaling pathways. It interacts with several proteins such as SMADs in the TGF-beta pathway contributing to the regulation of transcription and cellular responses to growth factors. In the Wnt signaling pathway PPP1CB deactivates key kinase members thereby modulating cell fate decisions and embryonic development. These pathways underline the phosphate turnover that PPP1CB controls impacting various cellular functions.
PPP1CB has been associated with cancer development and cardiovascular diseases. It contributes to oncogenesis when its regulatory role in cell cycle controls is disrupted leading to unchecked cell proliferation. Alterations in PPP1CB activity have also been linked to heart diseases where improper dephosphorylation affects muscle contraction and cardiac function. In cancer interactions with proteins such as PP2A alter the signaling balance while in heart disease proteins like PLN may connect to aberrant PPP1CB function emphasizing its critical role in maintaining cellular health.