Abcam, ab284704, Anti-PPP1A/PPP1CA (phospho T320) antibody [EP1512Y] - BSA and Azide free

Size: 100µg
Rabbit Monoclonal PPP1A/PPP1CA phospho T320 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP1512Y,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody detects PP1 alpha phosphorylated at Threonine 320.

Product details:
ab284704 is the carrier-free version of
ab62334
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Protein phosphatase 1 alpha also known as PPP1A or PPP1CA is a catalytic subunit of protein phosphatase 1 with a molecular weight of approximately 37 kDa. PPP1A plays a role in the dephosphorylation of serine/threonine residues in various proteins. It is expressed widely in human tissues with significant presence in the brain heart and skeletal muscle. PPP1A is essential for many cellular processes due to its widespread distribution and ability to interact with different substrate proteins.
Biological function summary
Protein phosphatase 1 alpha controls key aspects of cellular function. It often exists as part of complexes with other regulatory proteins which modulate its specificity and activity. These complexes participate in cell cycle regulation muscle contraction protein synthesis and glycogen metabolism. PPP1A's role in forming reversible phosphorylation networks highlights its importance in maintaining cellular homeostasis and responding to various signals.
Pathways
The role of PPP1A is vital in pathways such as the cell cycle and glycogen metabolism. It interacts with proteins like cyclin-dependent kinases to manage the cell division process and collaborates with glycogen synthase to regulate glycogen synthesis and breakdown. The involvement of PPP1A in these pathways showcases its versatility in influencing cellular metabolism and division processes.
Protein phosphatase 1 alpha is linked to cardiac diseases and cancer. Its dysregulation can influence heart muscle contractility contributing to cardiac hypertrophy and heart failure. Additionally abnormal PPP1A activity can impact tumorigenesis through interactions with cancer-related proteins like Akt. Understanding PPP1A's role in these diseases provides insights into potential therapeutic targets for treatment.