Abcam, ab284707, Anti-beta Dystroglycan antibody [EP2200Y] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal DAG1 antibody. Carrier free. Suitable for WB and reacts with Mouse, Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP2200Y,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human, Mouse,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab284707 is the carrier-free version of
ab62373
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Beta Dystroglycan also known as β-DG is a transmembrane protein with a molecular mass of approximately 43 kDa. It plays a mechanical role by linking the extracellular matrix to the cytoskeleton acting as a part of the dystrophin-glycoprotein complex. This protein is widely expressed in many tissues including skeletal muscle cardiac muscle and the central nervous system. Beta Dystroglycan serves as a receptor for components like laminin and agrin in the extracellular matrix.
Biological function summary
Beta Dystroglycan acts as a structural and signaling molecule within the dystrophin-glycoprotein complex. This complex stabilizes the sarcolemma during muscle contraction and helps in protecting muscle fibers from injury. Beta Dystroglycan also contributes to cellular signaling pathways by interacting with various intracellular proteins like dystrophin and alpha dystroglycan. It regulates cell adhesion and migration processes critical for tissue maintenance and repair.
Pathways
Beta Dystroglycan plays a critical role in the integrity of the dystrophin-associated glycoprotein complex. It is involved in the AKT signaling pathway which is essential for cellular growth and survival. Moreover beta Dystroglycan interacts closely with proteins such as dystrophin and utrophin facilitating their functions in muscle cells. Its interactions help maintain cell membrane stability and signal transduction processes important for normal cellular functions.
Beta Dystroglycan’s dysfunction has been closely linked to muscular dystrophies specifically Duchenne and Becker muscular dystrophies. These conditions involve muscle weakness and degeneration linked to abnormalities in the dystrophin-glycoprotein complex. Additionally aberrant glycosylation and signaling of beta Dystroglycan contribute to cancer progression through altered cell adhesion and migration often involving interactions with proteins like α-dystroglycan.