Abcam, ab284823, Anti-EPS15 antibody [EP9280] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Monoclonal EPS15 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP9280,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab284823 is the carrier-free version of
ab174291
This product has switched from a tissue culture supernatant (hybridoma) to Protein A purified (recombinant) 6th March 2025.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4 Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
EPS15 also known as Epidermal Growth Factor Receptor Substrate 15 is a critical adaptor protein involved in the process of endocytosis. The protein has a molecular mass of approximately 105 kDa and is highly expressed in various tissues particularly in the nervous system. EPS15 acts as an important player in clathrin-mediated endocytosis by interacting with components of endocytic machinery such as clathrin and AP2 adaptor complexes. It also contains multiple EH domains which facilitate protein-protein interactions needed for the internalization of receptors.
Biological function summary
The EPS15 protein contributes to the regulation of cell surface receptors and is involved in the recycling of these receptors back to the membrane or directing them to lysosomal degradation. This protein partakes in the formation of the clathrin-coated pits which are essential for the invagination of the membrane in the endocytic process. EPS15 works within a complex that aids in trafficking signals within cells allowing cells to maintain homeostasis by dynamically responding to external stimuli.
Pathways
EPS15 significantly influences the endocytosis and EGFR signaling pathways. The protein facilitates the downregulation of receptor tyrosine kinase signaling by mediating the internalization and recycling of the epidermal growth factor receptor (EGFR). EPS15 also interacts with other proteins such as Grb2 and Epsin which are integral to these pathways. The relationship between EPS15 and these pathways highlights its importance in cell communication and proliferation control.
Dysfunction of EPS15 links to cancer and neurological disorders. Abnormalities in EPS15 expression or function can contribute to cancer progression by disrupting EGFR signaling leading to unregulated cell growth and division. In neurological contexts altered EPS15 expression may affect synaptic transmission and faulty receptor trafficking potentially playing a role in neurodegenerative diseases. The interaction between EPS15 and proteins like EGFR and Epsin emphasizes its involvement in pathological conditions suggesting potential therapeutic targets for certain diseases.