Abcam, ab285264, Rat Alanine Aminotransferase (ALT) ELISA Kit

Size: 96Test
Rat Alanine Aminotransferase (ALT) ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Rat Alanine Aminotransferase (ALT) in Rat in Biofluids, Cell/Tissue Extracts samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Tissue Homogenate, Serum, Other biological fluids,
Reacts with:Rat,
Assay type:Sandwich (quantitative),
Sensitivity:< 0.469 ng/mL,
Range:0.78 - 50 ng/mL,
Assay Platform:Microplate (12 x 8 well strips)

Product details:
Alanine aminotransferase 1 mediates the control of a large number of enzymes, ion channels, aquaporins and other proteins through calcium-binding. Among the enzymes to be stimulated by the calmodulin-calcium complex are a number of protein kinases and phosphatases. Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.
This GPT ELISA kit (ab285264, E4325) is a sandwich ELISA assay for the quantitative measurement of rat GPT in serum, plasma and cell culture supernatants. The density of color is proportional to the amount of rat GPT captured from the samples
This product is manufactured by BioVision, an Abcam company and was previously called E4325 Alanine Aminotransferase 1 (GPT) (rat) ELISA Kit. E4325-100 is the same size as the 96 test size of ab285264.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Alanine transaminase also known as ALT or alanine aminotransferase is an important enzyme in the body. It has a molecular mass of approximately 54 kDa and catalyzes the conversion between alanine and pyruvate by transferring an amino group. ALT is mainly expressed in the liver but also present in other tissues like the heart and muscles. It is often measured as 'ALT activity' in clinical settings to ascertain liver health. In laboratories an 'ALT assay' or 'ALT kit' can determine alanine transaminase activity.
Biological function summary
Alanine transaminase plays a critical role in amino acid metabolism and energy production. It facilitates the transamination process which is significant for the balance of amino acids and carbohydrates. ALT as a standalone enzyme does not form part of a protein complex but works closely with alanine aminotransferase reactions. It helps maintain the cellular nitrogen balance linking nitrogen metabolism with energy pathways.
Pathways
The role of alanine transaminase is to interconnect carbohydrate and amino acid metabolism. It participates in the gluconeogenesis pathway and the citric acid cycle important for energy production. Through its activity in these pathways it interacts with proteins like aspartate aminotransferase (AST) affecting the balance of amino and keto acids in cells.
Alanine transaminase levels indicate liver conditions such as hepatitis and liver cirrhosis. Elevated ALT activity suggests liver damage or inflammation. It also connects to conditions involving muscular injury. In these scenarios increased presence and activity of AST often accompany ALT providing a broader picture of the extent of tissue damage. Understanding the involvement of ALT in these pathways helps diagnose and monitor the progression of relevant health issues.