Abcam, ab285267, Human Heparin ELISA Kit

Size: 96Test
Human Heparin ELISA Kit is a sandwich ELISA designed to quantify Human Heparin - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 9.3 - 300 ng/mL
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Tissue Homogenate, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Range:9.3 - 300 ng/mL,
Assay time:90m,
Assay Platform:Microplate (12 x 8 well strips)

Product details:
Human Heparin ELISA Kit ab285267 is a sandwich ELISA to measure Human Heparin in serum, plasma, cell culture supernatant, tissue homogenate.
How the assay works
The entire process can be performed within 90 minutes with only a single incubation and a wash step respectively, resulting in fewer handling steps, fewer errors and more consistent results.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Human Heparin ELISA Kit ab285267 protocol summary
1. Add standards or samples to appropriate wells. Incubate
2. Wash
3. Add HRP conjugate reagent to all wells. Incubate
4. Wash
5. Add Chromogen solutions to each well. Incubate.
6. Add stop solution and read at 450 nm.
This product is manufactured by BioVision, an Abcam company and was previously called E4464 QuickDetect™ Heparin (Human) ELISA Kit. E4464-100 is the same size as the 96 test size of ab285267.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Heparin also known as unfractionated heparin (UFH) is a glycosaminoglycan with a mass ranging from 3000 to 30000 Daltons. It is expressed in the granules of mast cells and released into the blood to exert its function. Heparin acts mechanically by binding to antithrombin a plasma protein significantly increasing its inhibitory effect on several activated clotting factors including thrombin and factor Xa. This binding results in modulation of the coagulation cascade which is critical for maintaining hemostasis in the body.
Biological function summary
Heparin plays an essential role in the regulation of blood coagulation acting as an anticoagulant. It does not function as part of a multi-protein complex yet its interaction with antithrombin involves a specific pentasaccharide sequence within heparin necessary for the enhancement of antithrombin's activity. This interaction prevents the formation of fibrin clots therefore enabling smooth blood circulation and reducing the risk of thrombosis.
Pathways
Heparin is deeply involved in the coagulation cascade and fibrinolysis. In the coagulation pathway it enhances the inhibitory effect of antithrombin on serine proteases like thrombin and factor Xa which play an important role in clot formation. Its action within this pathway indirectly affects the fibrinolysis pathway by regulating the formation of clots necessitating subsequent breakdown. The protein factor Xa is notably affected by the presence of heparin showcasing its role in these intricate biochemical pathways.
Heparin is significantly related to thrombosis and heparin-induced thrombocytopenia (HIT). It is used therapeutically to prevent and treat thromboembolic disorders given its ability to inhibit clot formation. However sometimes patients may develop HIT a condition where the immune system forms antibodies against the heparin-platelet factor 4 complex resulting in reduced platelet count and increased risk of thrombosis. The interaction of heparin with platelet factor 4 in this disorder highlights its complex role in disease mechanisms.