Abcam, ab285291, Mouse Estrogen ELISA Kit

Size: 96Test
Mouse Estrogen ELISA Kit is a Competitive ELISA used to quantify Mouse Estrogen with a sensitivity of 9.375 pg/ml - Colorimetric ELISA kit - 450 nm readout. Works on any standard plate reader - Wide dynamic range – 15.625 - 1000 pg/ml
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Tissue Homogenate, Cell culture supernatant, Serum, Other biological fluids,
Assay type:Quantitative,
Sensitivity:< 9.375 pg/mL,
Range:15.625 - 1000 pg/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Mouse Estrogen ELISA Kit is a competitive ELISA kit for the quantitative measurement of mouse estrogen with a sensitivity of 9.375 pg/mL
How the assay works
Mouse Estrogen ELISA kit an285291 is a competitive ELISA kit.
There are 2 types of competitive ELISAs:
- "antigen down": The sample antigen competes with a reference antigen for binding to a specific amount of labeled antibody. The reference antigen is pre-coated on a multi-well plate. The sample is added to the plate wells with a labeled antibody. Depending on the amount of antigen in the sample, more or fewer free antibodies will be available to bind the reference antigen. This means that the more of the antigen there is in the sample, the less reference antigen will be detected and the weaker the signal will be.
- "antibody down": the labeled antigen and the sample antigen (unlabeled) compete for binding to the primary antibody, which is immobilized on the plate. The lower the amount of antigen in the sample, the stronger the signal due to more labelled antigen in the well.
Assay specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility.
Mouse Estrogen ELISA kit ab285291 protocol summary
1. wash plate before adding standards, controls and samples
2. Add standard, samples and controls into appropriate wells. Incubate 45min
3. Wash
4. Add SABC solution into each well. Incubate for 30 min
5. Wash
6. Add TMB substrate into each well. Incubate for 15-30 min
7. Add stop solution. Read at 450 nm within 20 min.
How other researchers are using Mouse Estrogen ELISA kit ab285291
Mouse estrogen ELISA kit has been used to study the role of mitochondria dynamics in the female reproductive system in mice
and to study the mechanism of the NKB-NK3R pathway in Polycystic ovary syndrome (PCOS).
References
YP Budi et al. PMID 36765080
Fei Guo et al. PMID 36453600
Related and Recommended products
Mouse estrogen ELISA kit ab285291 is commonly used to study the reproductive system in mice. Another kit to assess reproductive hormones in mice is
- Mouse/Rat testosterone ELISA kit
ab285350
This product is manufactured by BioVision, an Abcam company and was previously called K4265 Estrogen (Mouse) ELISA Kit. K4265-100 is the same size as the 96 test size of ab285291.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C