Abcam, ab285341, Mouse Insulin ELISA Kit

Size: 96Test
Mouse Insulin ELISA Kit is a sandwich ELISA designed to quantify Mouse Insulin with a sensitivity of 46.9 pg/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 78 - 5000 pg/mL
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Tissue Homogenate, Cell culture supernatant, Serum, Other biological fluids, Cell Lysate,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:< 46.9 pg/mL,
Range:78 - 5000 pg/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Mouse Insulin ELISA Kit ab285341 is a sandwich ELISA to measure Mouse Insulin in serum, plasma, cell culture supernatant, cell lysate, tissue homogenate, other biological fluids with a sensitivity of 46.9 pg/ml.
How the assay works
The density of color is proportional to the amount of human Insulin captured from the samples.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Mouse Insulin ELISA Kit ab285341 protocol summary
1. Wash plate with wash solution
2. Add standards and samples into appropriate wells. Incubate then discard
3. Add Biotin detection antibody and incubate,
4. Wash
Add SABC solution and incubate
5. Wash
6. Add TMB solution and incubate
7. Add Stop solution and read at 450 nm
This product is manufactured by BioVision, an Abcam company and was previously called K4271 Insulin (Mouse) ELISA Kit. K4271-100 is the same size as the 96 test size of ab285341.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Insulin a peptide hormone plays an essential mechanical role in carbohydrate and fat metabolism. Insulin alternatively known as bovine insulin in some research contexts has a molecular mass of around 5800 Da. Beta cells in the pancreas express insulin where it gets secreted directly into the bloodstream. The hormone regulates glucose levels by facilitating the uptake of glucose into cells such as muscle and adipose tissues.
Biological function summary
Insulin influences several key metabolic processes by binding to its receptor a component of the insulin receptor substrate family. This triggers a cascade of events within cells promoting the uptake of glucose and converting it into energy. Insulin operates as a singular entity but plays a critical role in forming a complex with its receptor. This interaction triggers downstream effects that alter the activity of enzymes and transcription factors involved in glucose and lipid metabolism.
Pathways
Insulin is critical for the regulation of the PI3K-AKT signaling pathway and the MAPK pathway. These pathways modulate processes such as cell growth proliferation and survival. Insulin interacts closely with other proteins in these pathways such as the insulin receptor substrate proteins and influences downstream proteins like AKT kinase which has a significant role in mediating the metabolic effects initiated by insulin binding.
Insulin is most notably linked to diabetes mellitus characterized by insufficient insulin production or action. This deficiency disrupts normal glucose homeostasis leading to hyperglycemia. Other associated proteins include glucagon which counteracts insulin's role in lowering blood glucose levels and IRS-1 an important element through which insulin signaling is transduced. Researchers utilize mouse insulin ELISA and insulin ELISA kits to better understand these relationships and develop treatments for insulin-related disorders.