Abcam, ab285909, Recombinant human Clusterin protein (Active)

Size: 50µg
Recombinant human Clusterin protein (Active) is a Human Fragment protein, in the 23 to 449 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, FuncS, Size Exclusion Chromatography.
Key facts
Purity:>90% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag C-Terminus,
Applications:SDS-PAGE, Size Exclusion Chromatography, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:The chaperone activity of ApoJ was confirmed by its ability to bind to proteins under physical stress such as heat (e.g. Thyroglobulin) and chemical stress such as change in pH (e.g. Procathepsin K).,
Accession:P10909,
Animal free:No,
Carrier free:No,
Species:Human,
Reconstitution:Reconstitute in water,
Storage buffer:pH: 7.5Constituents: 0.58% Sodium chloride, 0.41% Sodium phosphate

Product details:
This product is manufactured by BioVision, an Abcam company and was previously called 7584 ApoJ/Clusterin, human recombinant. 7584-50 is the same size as the 50 µg size of ab285909.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Clusterin also known as Apolipoprotein J (ApoJ) is a glycoprotein with a mass of approximately 75-80 kDa. Scientists find clusterin expressed widely in the human body including the brain kidney and reproductive organs. This protein exists in two isoforms a secreted form and a nuclear form each with different locations and functions within the cells.
Biological function summary
Clusterin plays multiple roles particularly in lipid transport apoptosis and cell-cell interactions. It does not form part of a larger protein complex but it acts as a molecular chaperone that helps in folding and clearance of damaged proteins. This capability allows clusterin to protect cells against stress conditions such as oxidative stress by preventing abnormal protein aggregation.
Pathways
Clusterin is involved in pathways related to cell survival and lipid metabolism. It plays a role in the insulin signaling pathway and interacts with proteins like Heat Shock Protein 70 (HSP70) to assist in protein folding and in the activation of apoptotic pathways when needed. Clusterin's function in these processes helps in maintaining cellular homeostasis.
Scientists associate clusterin with Alzheimer’s disease and cancer progression. Elevated levels of clusterin have been observed in Alzheimer's patients suggesting a role in amyloid-beta plaque formation. In cancer clusterin expression influences tumor progression and resistance to therapies. Its interaction with other proteins such as Bax modulates the apoptotic pathways impacting cancer cell survival and chemotherapy resistance.