Abcam, ab286500, Human PRDM16 knockout HCT116 cell line

Size: 1000000Cells / vial
PRDM16 KO cell line available to order. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control available. Knockout. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HCT116,
Species or organism:Human,
Tissue:Colon,
Form:LiquidSee storage information,
Knockout validation:Next Generation Sequencing,Western blot,
Mutation description:Knockout,
Disease:Carcinoma

Product details:
Although we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.
Recommended control
: Human wild-type HCT116 cell line (ab288559). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PRDM16, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Next Generation Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PRDM16 also known as the PR-domain containing 16 is a transcription cofactor involved in the regulation of genes. It has a mass of approximately 140 kDa. This protein is found in multiple tissues with high expression levels observed in brown adipose tissue and skeletal muscle. PRDM16 regulates gene expression by interacting with other transcription factors and modifying chromatin structure.
Biological function summary
PRDM16 is essential in the determination of brown adipose tissue and skeletal muscle differentiation. It belongs to a complex that includes C/EBPβ and PPARγ which are pivotal for the conversion of myoblasts into thermogenic brown fat cells. This activity contributes to the generation of cells specialized for heat production which aids in maintaining body temperature.
Pathways
PRDM16 functions are linked to cellular metabolism and thermogenesis. It plays a substantial role in the Wnt/β-catenin signaling pathway where it influences energy balance. In this pathway PRDM16 interacts with Wnt signaling regulators and coactivators that control adipocyte differentiation and energy expenditure.
PRDM16 plays a role in myoblastic leukemia and obesity-related conditions. Changes in the function or expression of PRDM16 can contribute to pathological transformation leading to leukemia with proteins C/EBPβ and PPARγ also involved in related mechanisms. In obesity altered PRDM16 activity affects the formation and function of brown adipose tissue potentially disrupting normal energy metabolism and storage.