Abcam, ab286912, Anti-SARS-CoV-2 nsp14 antibody [EPR24839-87]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal R1AB antibody. Suitable for WB, I-ELISA and reacts with Recombinant fragment - SARS-CoV-2 samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR24839-87,
Isotype:IgG,
Carrier free:No,
Reacts with:SARS-CoV-2,
Applications:WB, I-ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SARS-CoV-2 nsp14 also known as non-structural protein 14 plays a mechanical role in the virus replication process. It functions as an exonuclease and methyltransferase enzyme contributing to RNA proofreading and mRNA cap modification. nsp14 is approximately 52 kDa in mass and is expressed as part of the viral replication-transcription complex in the infected host cell cytoplasm.
Biological function summary
Nsp14 contributes to the virus's ability to maintain the fidelity of RNA synthesis and evade host immune responses. It collaborates with other proteins like nsp10 as a cofactor to form a complex that enhances its exonuclease activity. This proofreading ability is essential for the coronavirus to minimize mutations during replication ensuring the production of viable viral progeny.
Pathways
Nsp14 is involved in important viral replication and immune evasion pathways. It participates in the viral lifecycle by proofreading nascent RNA and modifying the mRNA cap impacting the helicase nsp13 and messenger RNA methylation processes. The activities forming part of these pathways highlight the an essential role of nsp14 in the broader context of viral propagation and survival inside the host.
SARS-CoV-2 nsp14 is closely associated with COVID-19 the disease caused by the coronavirus. Its roles in RNA proofreading and immune evasion make it an important target for antiviral drug development aiming to combat the COVID-19 pandemic. nsp14's interactions with other non-structural proteins such as nsp10 and nsp13 further establish its importance in understanding virus-host interactions and developing therapeutic interventions against related coronavirus disorders such as SARS and MERS.