Abcam, ab28758, Anti-Cytochrome b reductase 1/DCYTB antibody

Size: 100µg
Goat Polyclonal Cytochrome b reductase 1/DCYTB antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CYBRD1 aa 250 to C-terminus.
Key facts
Host species:Goat,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human CYBRD1 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q53TN4

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide., Storage buffer-pH: 7.3Preservative: 0.02% Sodium azideConstituents: 0.5% BSA, 0.5% Tris, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Cytochrome b reductase 1 also known as DCYTB plays a mechanical role in electron transport. It is found in the duodenum and jejunum of the small intestine where it facilitates the reduction of ferric to ferrous iron. This protein weighs approximately 31 kDa. DCYTB integral to plasma membrane contains cytochrome b components that enable its redox function necessary for iron absorption.
Biological function summary
Cytochrome b reductase 1 influences iron homeostasis and is integral to the body's iron metabolism. DCYTB forms part of a complex responsible for the uptake and transport of dietary iron across the intestinal epithelium. This protein's activity augments during iron deficiency enhancing iron absorption to maintain balance. DCYTB works in conjunction with divalent metal transporter 1 (DMT1) to mediate iron entry into enterocytes.
Pathways
Cytochrome b reductase 1 takes part in the iron metabolism and absorption pathways. Its redox reactions convert dietary iron into a bioavailable form that DMT1 can transport. The iron homeostasis pathway heavily involves DCYTB emphasizing its relationship with proteins like ferroportin which exports iron from cells into circulation maintaining systemic iron levels.
Cytochrome b reductase 1 connects to anemia and iron overload disorders. Disrupted function or expression of DCYTB affects iron uptake potentially contributing to anemia when impaired or to iron overload in conditions of overexpression. DCYTB links to proteins such as hepcidin an iron-regulatory hormone further influencing systemic iron homeostasis and associated conditions.