Abcam, ab288198, Human SMARCA1 knockout HCT116 cell line

Size: 1000000Cells / vial
SMARCA1 KO cell line available to order. KO validated by. Free of charge wild type control available. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HCT116,
Species or organism:Human,
Tissue:Colon,
Form:LiquidSee storage information,
Disease:Carcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SMARCA1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SNF2L also known as SMARCA1 is a protein that functions as a chromatin remodeler. The molecular weight of SNF2L approximately 136 kDa. SNF2L is expressed widely with a higher presence in the brain and testis. The protein plays an active role in the regulation of gene expression by altering chromatin structure through ATP-dependent mechanisms. SNF2L belongs to the ISWI family of chromatin remodelers which facilitates the process by sliding or restructuring nucleosomes on DNA.
Biological function summary
SNF2L acts as part of the NURF (Nucleosome Remodeling Factor) complex. This complex is involved in modifying chromatin to make DNA accessible for transcription machinery. In addition to its role in transcription regulation SNF2L contributes to DNA replication and repair processes. By affecting chromatin architecture SNF2L influences the cell cycle and various developmental pathways. SNF2L's action is essential for maintaining proper gene expression patterns necessary for normal cellular function.
Pathways
SNF2L contributes to the Wnt signaling and neuronal differentiation pathways. In these contexts SNF2L works with proteins such as β-catenin in Wnt signaling where its chromatin remodeling abilities facilitate the transcription of Wnt target genes. Additionally SNF2L interacts with neural transcription factors during neuronal differentiation guiding the chromatin remodeling efforts necessary for neurogenesis. These pathways illustrate SNF2L's importance in regulating genes particularly those involved in cell fate decisions.
SNF2L has been associated with neurodevelopmental disorders and cancer. Mutations or dysregulation of SNF2L can lead to intellectual disabilities and other brain-related anomalies highlighting its significance in cognitive function. In cancer anomalies in the expression or mutation of SNF2L can influence tumor progression potentially through pathways involving other tumor-related chromatin remodelers like BRCA1. As a result understanding SNF2L can offer insights into therapeutic strategies for these conditions.