Abcam, ab288318, Substance P ELISA Kit

Size: 1 x 96Tests
Substance P ELISA Kit is a competitive ELISA designed to quantify Substance P with a sensitivity of 5.3 pg/mL - Colorimetric competitive ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 9.8 - 10000 pg/ml
Key facts
Detection method:Colorimetric,
Sample types:Saliva, Urine, Plasma, Cell culture supernatant, Serum,
Assay type:Competitive,
Sensitivity:= 5.3 pg/mL,
Range:9.8 - 10000 pg/mL,
Assay time:3h,
Assay Platform:Microplate

Product details:
Abcam's Substance P
in vitro
competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Substance P in biological fluids.
A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-Substance P antigen and a polyclonal rabbit antibody specific to Substance P. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of Substance P captured in the plate.
Substance P is a undecapeptide that displays a number of biological activities. The peptide was first discovered in 1931 by von Euler and Gaddum. They reported that extracts of equine brain and intestine contained a hypotensive and spasmogenic factor. The preparation, termed preparation P, was later found to be proteinaceous. The isolation and characterization of Substance P was carried out by Leeman's group in 1970. The structure is shown below.
Substance P: H-Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2
Substance P is synthesized in the ribosomes as a larger protein and then enzymatically converted into the active peptide. The peptide is widely distributed in the peripheral and central nervous systems of vertebrates, where it is thought to act as a neurotransmitter. In the peripheral system, Substance P is localized in the primary sensory neurons and neurons intrinsic to the gastrointestinal tract.
Cross Reactivity
Compound
% Cross Reactivity
Substance P
Substance P (4-11)
Substance P (7-11)
Substance P (3-11)
Physalaemin
Eledoisin
<0.001
α-Neurokinin
<0.001
β-Neurokinin
<0.001
Somatostatin
<0.001
Substance P (1-4)
<0.001

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Substance P also known as neurokinin 1 (NK1) receptor agonist is a neuropeptide with molecular mass approximately 1.3 kDa. Substance P exists mainly in the central nervous system and peripheral nervous system. This peptide performs mechanical roles by interacting with its receptor the NK1 receptor which is widespread in neurons and various tissues. It is important for processing pain signals and mood regulation. Substance P belongs to the family of tachykinins which are characterized by the common C-terminal sequence.
Biological function summary
This peptide acts as a neurotransmitter and neuromodulator in the body. Its functions are significant in the transmission of pain information into the central nervous system where it acts independently rather than being part of a larger protein complex. Additionally Substance P contributes to the regulation of inflammatory processes which impacts immune response. This peptide is not only important for pain perception but also participates in regulating emotional responses and stress.
Pathways
Substance P has a major role in the neurokinin signaling pathway. Within this pathway it interacts with other proteins such as calcitonin gene-related peptide (CGRP) enhancing its function in pain transmission and modulation. Additionally Substance P is part of the MAPK/ERK pathway where it influences cell proliferation and survival although its primary actions focus on signaling through the NK1 receptor.
Substance P is connected to conditions such as chronic pain and depression. By interacting with proteins like the NK1 receptor it is implicated in the sensation and perception of pain leading to an increased understanding of chronic pain mechanisms. Substance P also relates to inflammatory diseases with its involvement in pathways that influence inflammation and could serve as targets for therapeutic intervention in disorders like arthritis. Anti-substance therapeutics aim to modulate its effects to treat these conditions.