Abcam, ab288923, Human Thymidylate Synthase ELISA Kit

Size: 1 x 96Tests
Human Thymidylate Synthase ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Thymidylate Synthase with a sensitivity of 39.8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Cell culture extracts, Cell Lysate,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 39.8 pg/mL,
Range:85.938 - 5500 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Thymidylate Synthase ELISA Kit (ab288923) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Thymidylate Synthase in cell extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Thymidylate Synthase with 39.8 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Thymidylate Synthase (TS) also known as TYMS or TSase is an important enzyme involved in the de novo synthesis of thymidylate a nucleotide necessary for DNA replication and repair. TS catalyzes the methylation of deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP) using 510-methylenetetrahydrofolate as a cofactor. It has an approximate molecular weight of 36 kDa. The enzyme is expressed in the cytoplasm of proliferating cells predominantly in tissues with high cellular turnover like bone marrow and the intestinal lining.
Biological function summary
Thymidylate synthase plays a critical role in DNA synthesis and cell division. As a member of the folate cycle it indirectly influences the supply of thymidine for DNA synthesis affecting cell proliferation. The enzyme generally functions as a homodimer which is necessary for its activity. Increased expression of TS usually correlates with high rates of cell division making it an important factor in rapidly growing tissues and tumors.
Pathways
Several important biological networks involve thymidylate synthase especially the folate and pyrimidine metabolism pathways. These pathways are essential for the maintenance of nucleotide pools within the cell. TS is functionally related to proteins like dihydrofolate reductase (DHFR) which also play roles in folate metabolism and DNA synthesis. Collectively these proteins ensure the proper balance of nucleotide precursors for DNA replication and repair.
Thymidylate synthase is closely associated with cancer and certain autoimmunity conditions. Overexpression of TS is commonly observed in various cancers as tumor cells require increased nucleotide synthesis for rapid proliferation. This makes TS a target for several anticancer drugs like 5-fluorouracil which inhibits TS activity. Additionally aberrations in enzymes like dihydropyrimidine dehydrogenase (DPD) could affect TS function leading to chemotherapeutic agent sensitivity in cancer treatments.