Abcam, ab289153, Human PIWIL4 knockout HCT116 cell line

Size: 1000000Cells / vial
PIWIL4 KO cell line available to order. KO validated by. Free of charge wild type control available. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HCT116,
Species or organism:Human,
Tissue:Colon,
Form:LiquidSee storage information,
Disease:Carcinoma

Product details:
Although we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.
Recommended control
: Human wild-type HCT116 cell line (ab288559). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PIWIL4, Gene editing type-Knockout, Gene editing method-CRISPR technology, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PIWIL4 also known as PIWI-like 4 is a protein involved in RNA-mediated gene silencing with a molecular weight of approximately 97 kDa. It is expressed mostly in germ cell tissues including the testes and ovaries. PIWIL4 belongs to the PIWI subfamily of Argonaute proteins which is critical for the regulation of gene expression at the transcriptional and post-transcriptional levels.
Biological function summary
PIWIL4 plays an important role in the process of germ cell development and maintenance. It is essential for the formation of piRNA clusters and interacts with small non-coding RNA known as PIWI-interacting RNA (piRNA). It functions as part of a protein complex that facilitates the silencing of transposable elements and the regulation of gene expression therefore safeguarding genome integrity in germ cells.
Pathways
PIWIL4 integrates within the piRNA biogenesis pathway and RNA interference (RNAi) pathway key processes in cellular defense mechanisms. It associates with other PIWI family members such as PIWIL1 and PIWIL2 which collaborate to cleave and silence transposable elements and undesirable genetic elements. This function is critical in pausing transposition events that can disrupt genomic stability.
PIWIL4 has connections to certain cancers such as testicular germ cell tumor due to its role in regulating cell proliferation and genome protection. Abnormal expression or malfunction of PIWIL4 has implications in the development of infertility as it influences germ cell maturation. Investigations also assess its interactions with proteins like MAEL and TDRD1 which contribute to the maintenance of spermatogenic functions in testicular tissues.