Abcam, ab289642, Human Thyroxine Binding Globulin ELISA Kit

Size: 1 x 96Tests
Human Thyroxine Binding Globulin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Thyroxine Binding Globulin with a sensitivity of 8.624 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 8.624 pg/mL,
Range:18.75 - 1200 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Thyroxine Binding Globulin ELISA Kit (ab289642) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Thyroxine Binding Globulin in serum, plasma (heparin), plasma (EDTA), plasma (citrate), and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Thyroxine Binding Globulin with 8.624 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Thyroxine Binding Globulin (TBG) also called globulin 23 is a glycoprotein that binds thyroid hormones primarily thyroxine (T4) and triiodothyronine (T3). It facilitates the transport of these hormones through the bloodstream to various tissues. This binding globulin has a molecular weight of approximately 54 kDa. TBG is predominantly expressed in the liver and is an essential component within the circulatory system for hormone regulation. Although there is no substance called 'globulin green' TBG plays an important role in maintaining the systemic balance of thyroid hormones.
Biological function summary
The thyroxine-binding globulin helps maintain adequate levels of thyroid hormones in circulation ensuring a ready reserve supply to target tissues. TBG acts as a carrier protein and does not form part of a larger complex. It provides stability to the hormones and prevents their premature clearance from the bloodstream. Without it the availability and functionality of thyroid hormones within the body would be compromised affecting metabolic and growth functions.
Pathways
Thyroxine-binding globulin is critical in the thyroid hormone signaling pathway. This pathway regulates metabolism growth and development through the action of thyroid hormones. TBG is closely related to other hormone-binding proteins such as transthyretin and albumin which also participate in thyroid hormone transport albeit with different affinities. Its presence ensures that these hormones remain available for cellular uptake and action within various tissues influencing energy expenditure and developmental processes.
The function and levels of thyroxine-binding globulin have significant implications in conditions like hypothyroidism and hyperthyroidism. Altered TBG levels either due to genetic variations or external factors can lead to abnormal blood concentrations of thyroid hormones affecting growth and metabolism. In such disorders the interaction between TBG and thyroid hormones becomes critical. TBG levels can be influenced by other proteins like sex hormone-binding globulin (SHBG) which may reflect changes in the hormone-binding protein landscape during disease states contributing to dysregulated hormonal balance.