Abcam, ab289649, Human NECTIN1 ELISA Kit

Size: 1 x 96Tests
Human NECTIN1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human NECTIN1 with a sensitivity of 12.49 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:>= 12.49 pg/mL,
Range:34.375 - 2200 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human NECTIN1 ELISA Kit (ab289649) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human NECTIN1 in serum, hep plasma, EDTA plasma, Cit plasma and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human NECTIN1 with 12.49 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein PVRL1 also known as NECTIN1 or nectin-1 functions mechanically as an immunoglobulin-like cell adhesion molecule. It plays a role in creating adherens junctions between cells operating as both a homophilic and heterophilic binding partner. Nectin-1 has a molecular mass of approximately 97 kDa. It is expressed prominently in epithelial tissues and particularly in the nervous system which suggests it plays an important role in cell-cell communication in these areas.
Biological function summary
Nectin-1 facilitates cellular processes through its involvement in cell adhesion complexes. It functions as part of a larger complex including proteins like afadin which links to the actin cytoskeleton and promotes cell junction integrity. This organization impacts cellular adhesion migration and proliferation. Nectin-1's adhesive functions are fundamental in maintaining structural tissues and transmitting signals within cells a necessity for development and tissue maintenance.
Pathways
Nectin-1 contributes to the activation of the MAPK/ERK signaling pathway central to regulating various cell functions including proliferation and differentiation. It also participates in the cadherin-catenin complex pathway working in conjunction with E-cadherin a critical protein in maintaining epithelial cell layer integrity. These pathways highlight nectin-1’s role as a signal transducer affecting how cells interact and react to their environment.
Nectin-1 is implicated in cleft lip and palate disorder. Mutations affecting nectin-1 can disrupt cell adhesion processes necessary for proper development of facial structures. Furthermore alterations in nectin-1 gene expression are associated with susceptibility to herpes simplex virus infections as nectin-1 acts as a viral entry receptor. In these conditions its interaction with closely related membrane proteins such as nectin-3 is significant in disease manifestation and progression.