Abcam, ab290, Anti-GFP antibody

Size: 50µL / 500µL
Anti-GFP antibody (ab290) is a rabbit polyclonal antibody detecting GFP in Western Blot, IP, IHC-P, IHC-Fr, ICC/IF, ELISA, EM . - Over 3370 publications - Trusted since 2002 See more GFP antibodies and assay kits
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Applications:ICC/IF, ChIP, IHC-Fr, IP, EM, IHC-FrFl, ELISA, IHC-FoFr, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Aequorea victoria GFP.P42212,
Specificity:GFP antibody is reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, CFP, RFP and EGFP.

Product details:
Product Specifications
Anti-GFP antibody (ab290) is a rabbit polyclonal antibody and is validated for use in ELISA, EM, ICC/IF, IHC-FoFr, IHC-Fr, IHC-FrFl, IHC-P, IP and WB in human samples.
Anti-GFP antibody (ab290) was first used in a scientific publication in 2002 and has been cited over 3370 times in peer reviewed journals. It's performance in Western blot, immunofluorescence and IHC in human and mouse samples is trusted by the scientific community.
GFP antibodies are used to visualize proteins labelled with this tag in a variety of applications (for example imaging and Flow cytometry). To enable specific detection of your tagged protein, Anti-GFP antibody (ab290) has been validated in  ELISA, Western Blot and imaging applications.
Anti-GFP antibody (ab290) specifically detects GFP  (UniProt ID: P42212; Molecular weight: 27kDa) and is sold in a convenient trial size to enable initial testing (50 µL) and larger sizes for subsequent scaling up experiments (500 µL).
Quality and Validation
Abcam's high quality validation processes ensure Anti-GFP antibody (ab290) has high sensitivity and specificity.
Anti-GFP antibody (ab290) has 178 independent reviews from customers.
Target Information
Green Fluorescent Protein (GFP), originally derived from the jellyfish Aequorea victoria, emits a bright green fluorescence under ultraviolet or blue light. This unique property makes GFP an invaluable tool in molecular and cell biology research. Widely used to tag proteins, GFP allows scientists to visualize and track protein expression, localization, and interactions within living cells. Key applications of GFP include fluorescence microscopy (ICC, IHC, IF), flow cytometry (FC), and western blotting (WB).

Properties and Storage Information:
Form-Liquid, Purity-Whole antiserum, Purification notes-This antibody is provided as whole antiserum. It is not possible to determine the exact concentration of the anti-GFP antibody ab290, since whole serum contains many other host serum proteins and other antibodies, besides the antibody of interest. The total IgG concentration is 5 mg/mL.This antibody does not contain an anti-bacterial agent – use sterilized equipment and freeze small aliquots to reduce the risk of contamination., Storage buffer-Preservative: 0.05% Sodium azideConstituents: 1.25% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GFP also known as Green Fluorescent Protein acts as a bioluminescent marker derived from the jellyfish Aequorea victoria. GFP is popular in molecular biology for its fluorescence properties making it useful for visualizing proteins. This protein has a molecular weight of approximately 27 kDa. Researchers and scientists often express GFP in various organisms as a luminescent tag helping them observe protein expression localization and interaction within cells. GFP tagging involves the fusion of GFP to a protein of interest enabling the study of the protein's function and dynamics without affecting the host cell.
Biological function summary
GFP serves as a marker due to its ability to emit green fluorescence without requiring additional substrates or cofactors. GFP does not function within complexes like other proteins but acts as a standalone tool to monitor physiological processes. Scientists utilize techniques such as Western blot ELISA and microscopy along with GFP to track and quantify proteins inside living cells. Anti-GFP antibodies can detect GFP fusion proteins in various applications providing valuable insights into protein behavior and allowing robust assays involving GFP.
Pathways
GFP itself does not participate actively in traditional biochemical or signaling pathways. Instead it enables visual tracking within pathways. Researchers utilize GFP to study pathways like MAPK/ERK and PI3K/AKT where they track proteins related to these pathways using GFP tagging. For instance fusing GFP with proteins like ERK1/2 allows tracking phosphorylation events and signal transduction in living cells leading to better understanding of cellular responses to different stimuli.
Researchers use GFP as a model to study gene expression and protein interactions under disease conditions. For example in neurological disorders GFP helps visualize neuronal pathways and protein aggregation processes. By tagging proteins such as amyloid precursor protein (APP) or tau with GFP scientists can study their role in Alzheimer's disease progression. Similarly GFP facilitates the investigation of cancer pathways allowing visualization of tumor-related proteins and helping researchers study how cancer cells grow and invade tissues supporting cancer research and therapy development.