Abcam, ab300115, Anti-ADNP antibody [EPR25434-4] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal ADNP antibody. Carrier free. Suitable for WB, IHC-P, IHC-Fr, ICC/IF, Flow Cyt (Intra), IP and reacts with Human, Mouse, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR25434-4,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human, Mouse, Rat,
Applications:Flow Cyt (Intra), WB, IHC-P, ICC/IF, IP, IHC-FrSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The ADNP protein also known as Activity-dependent neuroprotector homeobox is a multifunctional protein with a mass of about 123 kDa. ADNP is highly expressed in the brain but also present in other tissues including heart and kidney. It regulates neural development and synaptic plasticity by interacting with chromatin and participating in transcriptional activities. The protein plays a role in cell cycle regulation and is involved in neuroprotection against multiple insults.
Biological function summary
ADNP plays critical roles in the development of the nervous system and maintenance of neuronal function. As part of a chromatin remodeling complex known as SWI/SNF it modulates the expression of genes necessary for neurogenesis and neuronal survival. ADNP affects microtubule dynamics which are important for neuron structure and transport by regulating activity of associated proteins. These functions make ADNP influential in maintaining a healthy nervous system and protecting against neurodegenerative processes.
Pathways
ADNP is necessary for proper functioning in neuroprotective pathways and the regulation of gene expression pathways. It closely interacts with the proteins REST and HDAC2 in the regulation of transcription important for neuronal cell survival. The involvement in Wnt signaling pathway an important regulator of cell proliferation and differentiation highlights its importance in neural development and plasticity. ADNP also affects the activity of MAPK/ERK pathway which links it to the control of cell proliferation and survival.
ADNP mutations or dysregulations have been connected to neurodevelopmental disorders such as Helsmoortel-Van der Aa syndrome and Alzheimer's disease. In relation to Helsmoortel-Van der Aa syndrome disruptions in ADNP lead to intellectual disability and developmental delays due to impaired neural growth. In Alzheimer's disease changes in ADNP expression affect the stability and function of tau a protein involved in neurofibrillary tangles. These interactions underline ADNP's significance in neuropsychiatric disorders and potential as a therapeutic target.