Abcam, ab300155, Anti-MED12 antibody [EPR25211-36] (BSA and Azide free)

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal MED12 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR25211-36,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human, Mouse, Rat,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MED12 also known as mediator complex subunit 12 is a component of the transcriptional Mediator complex. It weighs approximately 250 kDa and is widely expressed in many tissues showing high transcriptional regulatory activity. MED12 collaborates with other subunits within the Mediator complex to transmit signals from regulatory DNA elements to RNA polymerase II facilitating gene expression regulation.
Biological function summary
MED12 functions as a core component of the larger Mediator complex which plays an important role in controlling RNA polymerase II-dependent genes. Through interactions with activators and repressors MED12 participates in chromatin modification and looping affecting gene transcription rates. It is enriched in tissues where fine control of gene expression is essential impacting growth differentiation and development processes.
Pathways
MED12 integrates into critical signaling pathways including the Wnt/β-catenin and Hedgehog pathways. In these pathways MED12 interacts with other proteins like β-catenin serving as a connecting node that influences the transcription of target genes essential for cell proliferation and differentiation. Such interactions help coordinate cellular responses to external signals and ensure proper pathway regulation.
MED12 mutations have been linked to disorders like Lujan-Fryns syndrome and uterine leiomyomas. In these contexts MED12 interacts with G protein-coupled receptors and other transcription factors such as β-catenin resulting in dysregulation that leads to the development of these conditions. Understanding the connections between MED12 and associated proteins provides insights into the underlying mechanisms of these pathologies.