Abcam, ab300409, Anti-Rbm15/OTT + RBM15B antibody [EPR25177-138] (BSA and Azide free)

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal RBM15B antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR25177-138,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human, Mouse, Rat,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Rbm15 also known as OTT and RBM15B are members of the RNA-binding motif protein family. Rbm15 has a molecular mass of approximately 101 kDa. Both proteins possess RNA-binding motifs that mediate their involvement in post-transcriptional regulation. Rbm15 and RBM15B are widely expressed in various tissues with notable expression in hematopoietic cells. They regulate RNA methylation by associating with the m6A methyltransferase complex influencing mRNA processing and nuclear export.
Biological function summary
Rbm15 and RBM15B play significant roles in hematopoiesis and embryonic development. They are known to be components of the m6A methylation complex which modifies adenosine residues on RNA affecting RNA stability and translation. This complex interaction enables them to participate in controlling cell differentiation and growth important in maintaining proper cellular function and development. Through their RNA binding ability they also influence splice site selection and alternative splicing of pre-mRNA which is essential in the generation of protein diversity.
Pathways
Both Rbm15 and RBM15B contribute significantly to the maintenance of the Notch signaling and Wnt/β-catenin signaling pathways. These pathways are central to cell fate determination proliferation and apoptosis. Rbm15 interacts with key proteins like Notch receptors promoting signal mediation and thereby affecting downstream gene expression. In the Wnt pathway RBM15B may interface with β-catenin to regulate transcriptional activity influencing cell cycle and differentiation processes alongside proteins such as LEF/TCF.
Rbm15 and RBM15B have connections with acute myeloid leukemia (AML) and developmental disorders. Rbm15 is known for its implication in AML through fusion with the MKL1 gene contributing to leukemogenesis by altering gene expression profiles. Abnormal m6A methylation patterns involving RBM15B can disrupt normal cell differentiation and have been linked to developmental anomalies. These proteins' interactions with disease-related proteins like MKL1 and altered pathways illustrate their importance in understanding disease mechanisms and highlight potential therapeutic targets.