Abcam, ab300510, Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal ALDH1L1 antibody. Carrier free. Suitable for IP, Flow Cyt (Intra), ICC/IF, IHC-Fr, IHC-P, Dot, WB and reacts with Mouse, Human, Rat, Recombinant full length protein - Mouse samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR25443-54,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WB, ICC/IF, Dot, IP, IHC-Fr, Flow Cyt (Intra), IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ALDH1L1 and ALDH1L2 also known as 10-formyltetrahydrofolate dehydrogenase isoform 1 and 2 are enzymes involved in folate metabolism. ALDH1L1 has a molecular mass of approximately 100 kDa and is prominently expressed in the liver and brain. ALDH1L2 is found mainly in the mitochondria across various tissues. Both enzymes catalyze the conversion of 10-formyltetrahydrofolate to tetrahydrofolate and carbon dioxide playing an important role in cellular metabolism.
Biological function summary
The ALDH1L1 and ALDH1L2 participate in the regulation of one-carbon metabolism which is essential for nucleotide biosynthesis and amino acid interconversion. ALDH1L1 functions as part of a complex with other enzymes involved in folate metabolism facilitating the transfer of one-carbon units. These enzymes assist in maintaining proper cellular levels of folate derivatives fundamental for methylation processes and DNA synthesis.
Pathways
ALDH1L1 and ALDH1L2 contribute to the folate cycle and the related methionine cycle. They participate alongside proteins such as methylenetetrahydrofolate reductase (MTHFR) and methionine synthase both of which play roles in homocysteine regulation. Proper functioning of these pathways is necessary for efficient methyl group transfer important for numerous methylation reactions within cells.
Impaired function of ALDH1L1 and ALDH1L2 is associated with metabolic disorders including hyperhomocysteinemia and certain types of cancer. Abnormal activity of these enzymes can cause disruptions in folate metabolism leading to elevated homocysteine levels which in turn are linked to cardiovascular diseases. Additionally studies suggest that alterations in folate pathway enzymes including MTHFR might influence cancer progression by affecting DNA methylation status and gene expression.