Abcam, ab300511, Anti-Glycosylated Robo4 antibody [EPR26283-158]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal ROBO4 antibody. Suitable for WB, IHC-P and reacts with Mouse samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR26283-158,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Robo4 is a glycoprotein and can be deglycosylated by Protein Deglycosylation MIX II (PMID: 19139490). This antibody does not recognize the deglycosylated form of Robo4. See WB image for details.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Glycosylated Robo4 also known as Roundabout homolog 4 or Magic Roundabout is a transmembrane receptor with a molecular mass of approximately 100 kDa. This protein is predominantly expressed in the endothelial cells of blood vessels. Glycosylation plays a role in its function and stability. Robo4's principal function is to mediate cellular communication and contribute to the regulation of angiogenesis by interacting with other proteins and extracellular matrices.
Biological function summary
Glycosylated Robo4 regulates endothelial cell migration and vessel stability. It is not part of a large complex but collaborates with other proteins to maintain balanced blood vessel growth and repair. This activity provides a means for the vascular system to respond appropriately to cues for new vasculature or stabilize existing vessels in reaction to physiological demands.
Pathways
Glycosylated Robo4 interacts within the angiogenesis signaling pathway and the Slit-Robo signaling pathway. Within these pathways Robo4 associates with proteins like Slit2 and recruits downstream signaling partners to mediate endothelial integrity and angiogenic responses. Its role ensures that both new vessel formation and remodeling of existing vessels are tightly regulated preventing abnormal sprouting or regression.
Glycosylated Robo4 shows connections to pathologies such as cancer and diabetic retinopathy. In the context of cancer Robo4's interaction with Slit2 and other signaling proteins affects tumor angiogenesis by preventing excessive vessel growth that tumors could exploit for nutrition and spread. Similarly in diabetic retinopathy dysregulation of Robo4 activity can result in aberrant vessel formation in the retina leading to vision complications.