Abcam, ab300527, Anti-NOTCH3 antibody [EPR25183-101]

Size: 20µL / 100µL / 1mL
Anti-NOTCH3 antibody [EPR25183-101] is a Rabbit Monoclonal antibody that is used in NOTCH3 Flow Cytometry (Intra), IHC-P, Western Blot. Suitable for Human samples. NOTCH3 is a cell-surface receptor that is widely expressed on vascular smooth-muscle cells. Mutations in the NOTCH3 gene are primarily reponsible for the development of CADASIL syndrome, a rare but devastating cerebrovascular disorder that provides an opportunity to understand the mechanisms underlying other types of small-vessel arteriopathies, vascular dementia, and the relationship between migraine and stroke. Therapeutic antibodies targeting NOTCH3 are being investigated for both CADASIL and a wide range of cancer types.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR25183-101,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab300527 does not react in WB with mouse, IHC-P with mouse and rat, ICC with mouse, and intracellular flow cytometry with mouse.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The NOTCH3 protein a significant player in the NOTCH signaling pathway is a transmembrane receptor involved in cell differentiation. Sometimes referred to as 'Notch3' this protein has a molecular weight of approximately 244 kDa. NOTCH3 is widely expressed in vascular smooth muscle cells and tissues such as arteries and lungs. The protein also plays an important role in the communication between adjacent cells because of its location on the cell surface.
Biological function summary
NOTCH3 influences various cellular processes including proliferation apoptosis and differentiation. NOTCH3 does not function alone it is part of a regulatory complex that mediates cellular context and fate decisions. The receptor is cleaved upon ligand binding to facilitate intracellular signaling. This signaling is critical in the development and maintenance of vascular integrity which highlights the protein's importance in both physiological and pathological contexts.
Pathways
NOTCH3 plays a role in the NOTCH signaling pathway essential for cell fate determination and the smooth muscle cell differentiation pathway. Ligands such as Jagged and Delta-like proteins trigger NOTCH3 activation. Upon activation the NOTCH3 intracellular domain translocates to the nucleus where it interacts with transcriptional regulators like RBPJ. This interaction modulates the expression of target genes influencing cell differentiation and proliferation.
NOTCH3 is implicated in Cerebral Autosomal Dominant Arteriopathy with Subcortical Infarcts and Leukoencephalopathy (CADASIL) a genetic vascular disorder. Mutations in the NOTCH3 gene result in the accumulation of the NOTCH3 protein within arterial walls leading to the characteristic symptoms of CADASIL. Additionally the protein has been linked to pulmonary arterial hypertension through shared pathways with proteins such as BMP2 and SMADs indicating its broader impact on vascular pathophysiology.