Abcam, ab300619, Anti-ARID1B antibody [EPR25408-34]

Size: 10µL-TRIAL / 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal ARID1B antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Rat, Mouse samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR25408-34,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Mouse, Rat,
Applications:ICC/IF, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ARID1B also known as BAF250b is a component of the SWI/SNF chromatin remodeling complex. This protein has a mass of approximately 250 kDa. ARID1B interacts with DNA to regulate gene expression by modifying the chromatin structure making it more accessible for transcription machinery. It is expressed in various tissues including the brain heart and skeletal muscle suggesting a role in diverse physiological processes.
Biological function summary
The protein participates in the regulation of transcription by altering the chromatin architecture. As part of the SWI/SNF complex ARID1B works alongside other proteins to control which parts of the genome are accessible for transcription. Its activity influences numerous cellular events like cell differentiation and proliferation. The SWI/SNF complex plays a significant role in embryonic development and tumor suppression.
Pathways
ARID1B affects several critical biological processes such as Wnt signaling and cell cycle regulation. The protein interacts with beta-catenin in the Wnt pathway affecting gene expression and cell fate decisions. Additionally ARID1B modulates the cell cycle directly by influencing the expression of cyclins and cyclin-dependent kinases proteins essential for cell cycle progression.
Mutations in the ARID1B gene have associations with neurodevelopmental conditions like Coffin-Siris syndrome and intellectual disabilities. The protein's malfunction can disrupt normal brain development through pathways involving CREBBP a known coactivator in transcription. Additionally ARID1B alterations appear in certain cancers often resulting in abnormal proliferation and differentiation in affected cells highlighting the importance of its regulatory functions in maintaining cellular health.